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Herein, a sensitive and selective sensor for biothiols based on colorimetric assay is reported. S-adenosyl-L-methionine (SAM) could induce the selective aggregation of unmodified gold nanoparticles (AuNPs) by electrostatic interaction. In the presence of biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), AuNPs prefer to react with… (More)
Using unmodified Au nanorods with enzyme-linkage reaction, a label-free colorimetric method for simple and convenient assay of DNA methylation is presented.
exonuclease, and the corresponding buffer solution were purchased from New England Biolabs. Ltd (Beijing, China). The oligonucleotides (P1-P4) were synthesized and purified by Invitrogen Co. Ltd (Shanghai, China). The methylated oligonucleotides (P5-P6) and DNA polymerase I were purchased from Takara Biotechnoly Co. Ltd (DaLian, China). Tetrachloroauric… (More)