Skip to search formSkip to main contentSkip to account menu
Rapid and Multiplexed MicroRNA Diagnostic Assay Using Quantum Dot-Based Förster Resonance Energy Transfer.
TLDR
Clinical applicability is demonstrated by sensitive multiplexed quantification of three miRNAs at low (2 to 10 nM) and varying concentrations in samples that contained up to 10% serum.
View on PubMed
Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning.
TLDR
Time-gated (TG) detection of a single terbium-donor-based Förster resonance energy transfer (FRET) pair can be used to selectively quantify low nanomolar concentrations of multiple DNAs or microRNAs in a single sample.
View on PubMed
Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen
TLDR
The results show that FRET based on QD acceptors is suitable for multiplexed and sensitive biomarker detection in clinical diagnostics.
View PDF
Quantum Dot-Based FRET Immunoassay for HER2 Using Ultrasmall Affinity Proteins.
TLDR
It is shown that very small histidine-tagged albumin-binding domain-derived affinity proteins (ADAPTs) can efficiently self-assemble to zwitterionic ligand-coated quantum dots (QDs) and outperform previously tested assays with antibodies, antibody fragments, and nanobodies.
View on PubMed
Influence of the Spatial Conformation of Charged Ligands on the Optical Properties of Gold Nanoclusters
Photoluminescent gold nanoclusters (Au NCs) were synthesized in one step in aqueous conditions using a mixture of glutathione and (mono or multivalent) glutathione-modified arginine peptides. By…
View via Publisher
A Rapid, Amplification-Free, and Sensitive Diagnostic Assay for Single-Step Multiplexed Fluorescence Detection of MicroRNA.
TLDR
A fully homogeneous multiplexed miRNA FRET assay that combines careful biophotonic design with various RNA hybridization and ligation steps is presented that provides a unique combination of performance parameters compared to state-of-the-art miRNA detection technologies.
View on PubMed
Nanobodies and Antibodies for Duplexed EGFR/HER2 Immunoassays Using Terbium-to-Quantum Dot FRET
TLDR
A systematic study of size, type, orientation, specificity, nonspecific binding, and cross-reactivity of antibodies conjugated to QDs for single and duplexed EGFR and HER2 immunoassays.
View via Publisher
Direct conjugation of antibodies to the ZnS shell of quantum dots for FRET immunoassays with low picomolar detection limits.
TLDR
A simple strategy to directly conjugate IgG, F(ab')2, and Fab antibodies via their endogenous disulfide groups directly to the inorganic ZnS shell of compact penicillamine-coated QDs will be highly advantageous for nanocrystal-based biosensing applications.
View on PubMed
Time-gated FRET nanoassemblies for rapid and sensitive intra- and extracellular fluorescence imaging
TLDR
Effective brightness enhancement by FRET from several Tb to the same QD, the use of low nanomolar concentrations, and the quick and sensitive detection void of FRET acceptor background fluorescence are important advantages for advanced intra- and extracellular imaging of biomolecular interactions.
View on Publisher
Conformational Details of Quantum Dot-DNA Resolved by Förster Resonance Energy Transfer Lifetime Nanoruler.
TLDR
The extensive experimental and theoretical analysis of 11 different distances/configurations of the same QD-DNA conjugate provided important information on DNA conformation on nanoparticle surfaces and will be an important benchmark for the development and optimization of DNA-nanobiosensors.
View on PubMed
...
...
By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy Policy, Terms of Service, and Dataset License