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NMR Detection of Structures in the HIV-1 5′-Leader RNA That Regulate Genome Packaging
TLDR
A nuclear magnetic resonance approach was developed that enabled direct detection of structural elements within the intact leader that are critical for genome packaging and support a packaging mechanism in which translation, dimerization, NC binding, and packaging are regulated by a common RNA structural switch. Expand
Structural determinants and mechanism of HIV-1 genome packaging.
TLDR
Efforts to identify the molecular determinants and mechanism of human immunodeficiency virus type 1 genome packaging are reviewed. Expand
Identification of a minimal region of the HIV-1 5'-leader required for RNA dimerization, NC binding, and packaging.
TLDR
This work generated HIV-1 5'-L RNAs containing mutations and deletions designed to eliminate substructures without perturbing the overall structure of the leader and examined effects of the mutations on RNA dimerization, NC binding, and packaging. Expand
Structure of the HIV-1 RNA packaging signal
TLDR
The structure shows how the 5′ leader binds to the HIV protein that directs packaging, how unspliced dimeric genomes are selected for packaging, and how translation is suppressed when the genome dimerizes. Expand
DHX9/RHA Binding to the PBS-Segment of the Genomic RNA during HIV-1 Assembly Bolsters Virion Infectivity.
TLDR
These studies demonstrate the structural basis for HIV-1 genomic RNA to recruit beneficial cellular cofactor to virions and support of progeny virion infectivity by RHA is attributable to structure-dependent binding at the PBS-segment of the HIV- 1 5'-UTR during virus assembly. Expand
Infectivity of SARS-CoV-2: there Is Something More than D614G?
TLDR
SARS-CoV2 has evolved by developing mutations in different viral proteins, and most strikingly, mutation P323L in nsp12 (an RNA-dependent RNA polymerase or RdRp) and D614G in S-protein co-evolved throughout the world. Expand
DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA
TLDR
It is demonstrated that the Bordetella phage DGR-encoded RT uses the 3′-OH of a nicked template RNA to initiate reverse transcription, during which random nucleotides are incorporated when adenine residues in the template are copied into complementary DNA (cDNA). Expand
Database proton NMR chemical shifts for RNA signal assignment and validation
TLDR
A computer program was developed that enables convenient comparison of RNA 1H NMR assignments with database predictions, which should facilitate future signal assignment/validation efforts and enable rapid identification of non-canonical RNA structures and RNA-ligand/protein interaction sites. Expand
Feasibility of Known RNA Polymerase Inhibitors as Anti-SARS-CoV-2 Drugs
TLDR
Using computational and bioinformatics tools, this work presents the feasibility of reported broad-spectrum RNA polymerase inhibitors as anti- SARS-CoV-2 drugs targeting its mainRNA polymerase, suggesting that investigational and approved nucleoside RNA polymerases inhibitors have potential asAnti-SARS- CoV- 2 drugs. Expand
Virion-associated, host-derived DHX9/RNA helicase A enhances the processivity of HIV-1 reverse transcriptase on genomic RNA
TLDR
This study identifies RHA as a processivity factor of HIV-1 RT, and indicates that RHA in HIV- 1 virions is required for the efficient catalysis of (−)cDNA synthesis during viral infection before capsid core uncoating. Expand
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