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ESTIMATION OF MICROBIAL PROTEIN SUPPLY TO SHEEP AND CATTLE BASED ON URINARY EXCRETION OF PURINE DERIVATIVES - AN OVERVIEW OF THE TECHNICAL DETAILS
- X. B. Chen, M. J. Gomes
- Biology
- 1995
Introduction 1 Principle of the method 1 Brief background of purine metabolism in ruminants 2 Limitation of the method 5 Sample collection 5 Determination of purine derivatives 6 Dilution of urine… Expand
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Excretion of purine derivatives by ruminants : effect of microbial nucleic acid infusion on purine derivative excretion by steers
- J. Verbič, X. B. Chen, N. Macleod, E. Ørskov
- Biology
- 1 June 1990
Two steers totally nourished by intragastric infusion of volatile fatty acids and casein were given an abomasal infusion of a microbial protein preparation (Pruteen) at eight rates with a purine… Expand
Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep.
- C. Newbold, R. Wallace, X. B. Chen, F. Mcintosh
- Biology, Medicine
- Journal of animal science
- 1 June 1995
A ruminal simulation device (Rusitec) was used to compare the effects of Saccharomyces cerevisiae strains NCYC 240, NCYC 694, NCYC 1026, NCYC 1088, and Yea-Sacc (a commercial product containing S.… Expand
Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep.
- X. B. Chen, F. Hovell, E. Orskov, D. Brown
- Biology, Medicine
- The British journal of nutrition
- 1990
The present study examined the relationship between the supply of exogenous nucleic acid (NA) purines and their recovery as the derivatives hypoxanthine, xanthine, uric acid and allantoin in urine.… Expand
The effect of feed intake and body weight on purine derivative excretion and microbial protein supply in sheep.
- X. B. Chen, Y. Chen, M. Franklin, E. Orskov, W. Shand
- Biology, Medicine
- Journal of animal science
- 1 May 1992
Urinary excretion of purine derivatives (PD) was used to estimate the microbial N (MN) supply to sheep in three experiments designed to examine the effects of DMI and BW on the efficiency of… Expand
Excretion of purine derivatives by ruminants: endogenous excretion, differences between cattle and sheep.
- X. B. Chen, E. Orskov, F. Hovell
- Biology, Medicine
- The British journal of nutrition
- 1990
The endogenous urinary excretion of the purine derivatives allantoin, uric acid and xanthine plus hypoxanthine were measured in twenty-nine lambs, ten cattle (six steers, one cow and three… Expand
Evaluation of the use of the purine derivative: creatinine ratio in spot urine and plasma samples as an index of microbial protein supply in ruminants: studies in sheep
- X. B. Chen, A. Mejía, D. Kyle, E. Ørskov
- Chemistry
- 1 August 1995
In ruminants, the urinary excretion of purine derivatives (PD) reflects the absorption of microbial purines and can be used as an index of microbial protein supply. The objective of this study,… Expand
Effect of feeding frequency on diurnal variation in plasma and urinary purine derivatives in steers
- X. B. Chen, G. Grubić, E. Ørskov, P. Osuji
- Biology
- 1 October 1992
The diurnal changes in plasma concentration and urinary excretion of purine derivatives (PD) were examined in four steers given their food in either one or two meals daily in two 18-day periods. In… Expand
Measurement of allantoin in urine and plasma by high-performance liquid chromatography with pre-column derivatization.
- X. B. Chen, D. Kyle, E. Orskov
- Chemistry, Medicine
- Journal of chromatography
- 11 August 1993
A method is reported for determination of allantoin in urine and plasma based on high-performance liquid chromatography (HPLC) and pre-column derivatization. In the derivatization procedure,… Expand
Urinary excretion of purine derivatives and tissue xanthine oxidase (EC 1.2.3.2) activity in buffaloes (Bubalis bubalis) with special reference to differences between buffaloes and Bos taurus cattle.
- X. B. Chen, L. Samaraweera, D. Kyle, E. Orskov, H. Abeygunawardene
- Biology, Medicine
- The British journal of nutrition
- 1 March 1996
The urinary excretion of purine derivatives (PD) was measured in six buffaloes (Bubalis bubalis) during fasting and in fourteen buffaloes given four restricted levels of roughage (2.5-4.8 kg DM/d).… Expand
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