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During poxvirus infection, both viral genomes and transfected DNAs are converted into high-molecular-weight concatemers by the replicative machinery. However, aside from the fact that concatemer formation coincides with viral replication, the mechanism and protein(s) catalyzing the reaction are unknown. Here we show that vaccinia virus DNA polymerase can(More)
Load Forecasting plays a critical role in the management, scheduling and dispatching operations in power systems, and it concerns the prediction of energy demand in different time spans. In future electric grids, to achieve a greater control and flexibility than in actual electric grids, a reliable forecasting of load demand could help to avoid dispatch(More)
Ab initio density functional theory (DFT) calculations are performed to study the adsorption of H2 molecules on a Ti-doped Mg(0001) surface. We find that two hydrogen molecules are able to dissociate on top of the Ti atom with very small activation barriers (0.103 and 0.145 eV for the first and second H2 molecules, respectively). Additionally, a molecular(More)
This paper investigates the Periodic Capacitated Arc Routing Problem (PCARP), which is often encountered in the waste collection application. PCARP is an extension of the well-known Capacitated Arc Routing Problem (CARP) from a single period to a multi-period horizon. PCARP is a hierarchical optimization problem which has a primary objective (minimizing the(More)
We have examined mechanisms of recombination in mammalian cells infected with herpes simplex virus type 1 (HSV-1). Amplification of plasmids containing a viral origin of replication, oriS, in cells superinfected with HSV-1 revealed that linear DNA could be efficiently converted to templates for replication. Two distinct pathways were observed: imprecise end(More)
Replicating poxviruses catalyze high-frequency recombination reactions by a process that is not well understood. Using transfected DNA substrates we show that these viruses probably use a single-strand annealing recombination mechanism. Plasmids carrying overlapping portions of a luciferase gene expression cassette and luciferase assays were first shown to(More)
Cellular topoisomerase II is specifically inactivated by the drug ICRF-193. This compound turns topoisomerase II into a closed clamp that is unable to cleave DNA. We have investigated the effects of this inhibitor on the replication of herpes simplex virus type 1. We show that ICRF-193 at low multiplicities of infection dramatically inhibits viral DNA(More)
Homologous recombination was examined in cells infected with herpes simplex virus type I. Circular and linear DNA with directly repeated sequences was introduced as recombination substrates into cells. Recombination was measured either by origin-dependent amplification of recombination products or by recombination-dependent expression of luciferase from a(More)
— Online service provision is becoming increasingly decentralized as system designers pursue the benefits gained from utilizing nodes at the periphery of the network. However , distributing control means relying on the cooperation of participating agents, and it is a significant challenge to design mechanisms that incentivise optimal global behavior in a(More)