Learn More
OBJECTIVE To investigate the role of N-cadherin and fibronectin during chondrogenesis. METHODS Immunohistochemical method and autibody induced changes of aggregation of cells were used to assay the expressions of N-cadherin and fibronectin during cell differentiation. RESULTS The N-cadherin was present in the area of the cell nodular area in the 24(More)
BACKGROUND In general the traditional static seeding method has its limitation while the dynamic seeding method reveals its advantages over traditional static method. We compared static and dynamic seeding method for human bone marrow stromal cells (hBMSCs) in bone tissue engineering. METHODS DNA assay was used for detecting the maximal initial seeding(More)
BACKGROUND AND PURPOSE Patients with neuropsychiatric systemic lupus erythematosus have worse outcomes compared with those with systemic lupus erythematosus. A better understanding of the mechanisms of neuropsychiatric systemic lupus erythematosus could potentially improve diagnosis and management. The goal of this study was to investigate the differences(More)
OBJECTIVE To evaluate the cartilage formation ability of allogeneic mesenchymal stem cells implanted into sheep joint cavity without the use of immunosuppressive therapy. METHODS Allogeneic mesenchymal stem cells (MSCs) loaded onto porous beta-tricalcium phosphate ceramic (beta-TCP) were implanted into normal sheep joint cavity. A complete mismatch(More)
OBJECTIVE To study the effects of BMP-2 gene therapy on vascularization in repairing bone defects. METHODS The isolated rabbit mesenchymal stem cells (rBMSC), after being transfected by adenovirus carrying BMP-2 gene (Ad-BMP-2) and seeded on xenogeneic bone scaffolds, were used to repair 1.5 cm-long radius bone defects. Five methods were in use in the(More)
OBJECTIVE To label the primary articular chondrocytes overexpressing human insulin-like growth factor (hIGF 1) with green fluorescent protein (GFP) for repair of articular cartilage defects in rabbits. METHODS GFP cDNA was inserted into pcDNA3.1 hIGF 1 to label the expression vector. The recombinant vector, pcGI, a mammalian expression vector with(More)
to a decrease in the density of presynaptic Ca 2þ channel clusters. It is conceivable that BRP tightly surrounds but is not part of the T-bar structure, contained within the unlabeled center of donuts. BRP may establish a matrix, required for both T-bar assembly as well as the appropriate localization of active zone components including Ca 2þ channels,(More)
OBJECTIVE To label human insulin-like growth factor-I (hIGF-I) eukaryotic expression vector with green fluorescent protein (GFP) for the repair of articular cartilage defects. METHODS GFP cDNA was inserted into pcDNA(3.1)-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus(More)
OBJECTIVE To illustrate the effect and complication of orthopedic applications for biodegradable and absorbable internal fixation of fractures, and to indicate the existent problem and research aspect currently. METHODS The recent literatures on orthopedic applications and study of biodegradable and absorbable internal fixation for fractures were(More)