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In contrast to the success achieved with the production of hybridomas in the mouse system, creating human hybridomas is problematic. The reason is believed to be a lack of suitable malignant human cell lines. The work presented here demonstrates the establishment of three human parent cell lines--two of which are of T cell origin--by installing hypoxanthine(More)
Studies on subjective body odour ratings suggest that humans exhibit preferences for human leucocyte antigen (HLA)-dissimilar persons. However, with regard to the extreme polymorphism of the HLA gene loci, the behavioural impact of the proposed HLA-related attracting signals seems to be minimal. Furthermore, the role of HLA-related chemosignals in same- and(More)
To obtain further insight into the informatory possibilities of the Takasugi-Klein microcytotoxicity test (MCT), mouse spleen cells were prepared after a single immunization with 5 X 10(7) H-2 incompatible cells and tested for reactivity against mouse fibroblasts. The following three aspects were investigated: (1) Specificity of the reaction: various(More)
Clinical data have suggested that graft-versus-host disease (GVHD) plays a crucial role in the antileukemic effects of bone marrow grafts. We investigated (a) whether bone marrow cells unable to induce GVHD can effect graft-versus-leukemia (GVL) activity and (b) whether such antileukemic capacity depends on the presence of T lymphocytes in the graft. Balb/c(More)
Recombinant adeno-associated virus (rAAV) is a replication-defective parvovirus which is being explored as a vector for gene therapy because of its broad host range, excellent safety profile, and durable transgene expression in infected hosts. rAAV has also been reported by several groups to induce little or no immune response to its encoded transgene(More)
To clarify contradictory information in the literature about the immunogenic effect of the trachea, tracheal transplantations were performed orthotopically and heterotopically in two combinations of inbred rat strains. In all in vivo experiments it was possible to demonstrate a considerable systemic immunisation by transplantation antigens. There were no(More)
To determine what influence the extent of genetic difference and degree of presensitization exercise on reactions to tracheal transplants, orthotopic tracheal transplantations were performed in 189 rats. In groups with minor genetic differences (non-RT1-different combination) and major genetic differences (RT1-different combination), the survival rates were(More)
Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IRI measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELISA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle(More)
Besides its immunological function of self/non-self discrimination the major histocompatibility complex (MHC) has been recognized as a possible source of individual specific body odors. Dating back to speculations on the role of the extraordinary polymorphism of the MHC as background of an individual chemosensory identity and to early observations of(More)
To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class II monoclonal antibody (mAb). How effectively this procedure blocked class II-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions(More)