Wolfgang G. W. Kurz

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A procedure for cryogenic storage of alkaloid producing cell lines of periwinkle, Catharanthus roseus (L.) G. Don., has been developed. The procedure differs from established cryopreservation protocols in several aspects. Specifically, 4-day-old suspension subcultures of three cell lines were precultured in nutrient media supplemented with 1 molar sorbitol(More)
The activities of the following enzymes were studied in connection with dinitrogen fixation in pea bacteroids: glutamine synthetase(L-glutamate: ammonia ligase (ADP-forming)(EC 6.3.1.2)(GS); glutamate dehydrogenase (NADP+)(L-glutamate: NADP+ oxidoreductase (deaminating)(EC 1.4.1.4)(GDH); glutamate synthase (L-glutamine: 2-exeglutarate aminotransferase(More)
When added to suspension cultures of CATHARANTHUS ROSEUS, abscisic acid (ABA) stimulated intracellular accumulation of the indole alkaloids catharanthine and ajmalicine in both flask and 30 litre fermenter-scale systems. The response varied, and depended upon the cell line, the concentration and source of the ABA, and the growth phase at which the cells(More)
Callus derived from hypocotyls of periwinkle, Catharanthus roseus, responded to culture on nutrient media supplementedwith IAA, BA, and zeatin with shoot formation at low frequencies. However, shoot regenerating callus could be very successfully propagated and subcultured. Alkaloid profiles of callus derived from the original explants (hypocotyls) as well(More)
Opium poppy laticifers are internal secretory cells which form a complex network of latex vessels through the gradual disappearance of their common cell walls [4]. Laticifer cytoplasm or latex is under positive osmotic pressure so that it covers wound surfaces when the plant is injured. Once exposed to air poppy latex dries into the brown, sticky gum called(More)
Developing seedlings of Catharanthus roseus were analyzed for appearance of tryptophan decarboxylase (TDC), strictosidine synthase (SS), N-methyltransferase (NMT) and O-acetyltransferase (DAT) enzyme activities. SS enzyme activity appeared early after germination and was present throughout most of the developmental study. TDC activity was highly regulated(More)
Dirofilariasis due to Dirofilaria repens is a rare anthropozoonotic disease, with dogs and cats being the definite hosts for this parasite. This worm is transmitted by various species of mosquitoes and usually presents as a subcutaneous nodule. Human cases have been reported from all around the Mediterranean, Eastern Europe, and Central and Southern Asia.(More)
Eleven different plant cell tissue cultures of both legume and non-legume origin have been grown in direct association, and in separate but close proximal association with both Spirillum lipoferum and Rhizobium sp. 32H1. Basic similarities were found in the nutritional requirement for the induction of nitrogenase activity (C2H2) in both organisms. In the(More)
Habituated and tumorous Catharanthus roseus cells grown in the absence of hormones accumulated indole alkaloids. Total alkaloids and alkaloid pattern were the same when cells were cultured in medium without hormones or in alkaloid production medium with and without indole acetic acid. Treatment of cells with Pythium homogenate as elicitor did not increase(More)