Witold Postek

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We demonstrate an integrated system for rapid and automated generation of multiple, chemically distinct populations of ~10(3)-10(4) sub-nanoliter droplets. Generation of these 'libraries of droplets' proceeds in the following automated steps: i) generation of a sequence of micro-liter droplets of individually predetermined composition, ii) injection of(More)
We demonstrate a microfluidic system for the precise (coefficient of variance between repetitions below 4%) and highly accurate (average difference from two-fold dilution below 1%) serial dilution of solutions inside droplets with a volume of ca. 1 μl. The two-fold dilution series can be prepared with the correlation coefficient as high as R2 = 0.999. The(More)
We demonstrate an integrated system (fig.1) for rapid and automated generation of libraries comprising multiple (~10-10) populations of sub-nanoliter droplets. Each population has a distinct chemical composition. Protocol of generation of libraries that we report comprises i) generation of a sequence of micro-liter droplets by aspiration of small portions(More)
We present a novel geometry of microfluidic channels that allows us to passively generate monodisperse emulsions of hundreds of droplets smaller than 1 nL from collections of larger (ca. 0.4 μL) mother droplets. We introduce a new microfluidic module for the generation of droplets via passive break-up at a step. The module alleviates a common problem in(More)
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