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Purpose – This paper attempts to understand the reasons for knowledge management (KM) project failure. Design/methodology/approach – Five well-documented cases of KM project failure in the current literature are reviewed. For each case, the authors examine the circumstantial elements of the failure, including the rationale and intended objectives of the KM(More)
In a test suite, all the test cases should be independent: no test should affect any other test’s result, and running the tests in any order should produce the same test results. Techniques such as test prioritization generally assume that the tests in a suite are independent. Test dependence is a little-studied phenomenon. This paper presents five(More)
Ca(2+) signals regulate cell proliferation, but the spatial and temporal specificity of these signals is unknown. Here we use selective buffers of nucleoplasmic or cytoplasmic Ca(2+) to determine that cell proliferation depends upon Ca(2+) signals within the nucleus rather than in the cytoplasm. Nuclear Ca(2+) signals stimulate cell growth rather than(More)
L-nucleoside analogs are a new class of antiviral and anticancer agents, several of which are currently used in the clinic. The phosphorylation of these agents to the triphosphate form is thought to be important for exertion of their pharmacological activities. 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methyluracil (L-FMAU; Clevudine) is a thymidine(More)
A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3' → 5' exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time(More)