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Washed human spermatozoa had an endogenous oxygen uptake of 2.14 +/- 0.17 nmol O2/10(8) spermatozoa/min (mean +/- s.e..m., n = 35) which was stimulated by succinate (Vmax = 9.64 +/- 0.44 nmol O2/10(8) spermatozoa/min) but not by other substrates. The ATP concentration in freshly washed spermatozoa was 12.18 +/- 0.54 (s.e.m.) nmol/10(8) spermatozoa (n = 26)(More)
Male rats given 6-chloro-6-deoxyglucose (240 mg/kg/day for 28 days) developed spermatocoeles in their ductuli efferentes or caput epididymides. They had a lower serum triglyceride content than controls (0.87 +/- 0.19 vs 1.84 +/- 0.19 mM, Mean +/- SEM; n = 6) and gained less weight (2.55 +/- 0.37 vs 4.1 +/- 0.96 g/day, Mean +/- SEM; n = 6). There was no(More)
6-Chloro-6-deoxyglucose, 6-chloro-6-deoxymannose, 6-chloro-6-deoxy-fructose, 6-chloro-6-deoxyglucitol, 6-chloro-6-deoxygalactose and (S) alpha-chlorohydrin all produced spermatocoeles in the ductuli efferentes and epididymis of the rat and were neurotoxic in the mouse, but only alpha-chlorohydrin caused substantial inhibition of glucose metabolism in bull(More)
It has been suggested that human spermatozoa contain an NADPH oxidase that could generate reactive oxygen species involved in signalling pathways to promote fertility. The proposal depends on observations that the addition of NADPH to purified human spermatozoa stimulates chemiluminescence by the superoxide (O2-) probe, lucigenin. We confirmed these(More)
Certain chlorine-substituted sugars with chemical similarities to sucralose have been demonstrated previously to diminish or inhibit sperm glycolysis and fertility in the rat ([Ford]). In order to investigate this potential for sucralose, epididymal spermatozoa were recovered from rats exposed in vivo to oral doses of one of three of these substituted(More)
[U-14C]Sucrose and D-[1-14C]mannitol were used to determine the tritiated water space of human spermatozoa to validate these compounds as markers for the extracellular space. Calculations based on 0.03 mM-[U-14C]sucrose gave a negative water space. The water space estimated with 0.03 mM-D[1-14C]mannitol was unstable but a stable result was obtained with 0.3(More)
6-chloro-6-deoxyglucose (24 mg/kg/day) produces infertility in male rats and marmosets. In the present study very high doses (480 mg/kg/day) were found not to impair the fertility of male mice. However, such doses induced the formation of vacuolated lesions in specific regions of central nervous system grey matter causing varying degrees of physical(More)
The concentrations of calcium and citrate in 52 samples of human semen obtained from 10 donors were 11.07 +/- 0.41 (mean +/- SEM) and 27.2 +/- 1.62 mM, respectively, and were strongly correlated (r = + 0.79). The pH of the semen was 7.65 +/- 0.069. All 3 parameters varied significantly between the donors (P less than 0.001 in each case), but the activity of(More)
Male rats given daily doses of 240 mumol 6,6'-dichloro-6,6'-dideoxysucrose or 6'-chloro-6'-deoxysucrose or 6-chloro-6-deoxysucrose/kg for 28 days became infertile after 7 days and remained infertile during treatment. Fertility was partly recovered 3 weeks after the last dose and completely restored by 6 weeks. 6-Chloro-6-deoxyglucose and(More)