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Using in silico analysis we studied a novel family of repetitive DNA sequences that is present among both domains of the prokaryotes (Archaea and Bacteria), but absent from eukaryotes or viruses. This family is characterized by direct repeats, varying in size from 21 to 37 bp, interspaced by similarly sized non-repetitive sequences. To appreciate their(More)
In this study, the occurrence of persistent intramammary infections caused by Escherichia coli with recurrent episodes of clinical mastitis caused by E. coli are described for a cohort of 300 Dutch dairy herds. Calculations on the recurrent episodes were based on data collected by dairy farmers. The genotype of the E. coli strains was determined by means of(More)
Four G adhesins, cloned from uropathogenic Escherichia coli strains, were examined for binding to glycolipids and various eukaryotic cells. PapGAD110 and PapGIA2 showed virtually identical binding patterns to Gal alpha 1-4Gal-containing glycolipids, while PapGJ96 differed slightly and PrsGJ96 markedly with respect to the effect of neighbouring groups on the(More)
Escherichia coli strains bind to Gal alpha 1-4Gal-containing glycolipids via P pili-associated G-adhesins. Three functional classes of adhesins with different binding specificities are encoded by conserved G-alleles. We suggest that the Class I papG-allele of strain J96 is a novel acquisition possibly introduced via horizontal gene transfer into one of the(More)
BACKGROUND The objective was to investigate the phenotypic and genotypic resistance and the horizontal transfer of resistance determinants from Salmonella isolates from humans and animals in Vietnam. METHODOLOGY/PRINCIPAL FINDINGS The susceptibility of 297 epidemiologically unrelated non-typhoid Salmonella isolates was investigated by disk diffusion(More)
The accurate performance of antimicrobial susceptibility testing of bacteria from animal sources and the correct presentation of the results is a complex matter. A review of the published literature revealed a number of recurring errors with regard to methodology, quality control, appropriate interpretive criteria, and calculation of MIC(50) and MIC(90)(More)
Despite the presence high levels of Arcobacter spp. on chicken carcasses, the source of arcobacter contamination in slaughterhouses still remains unclear. It has been hypothesised in the literature that Arcobacter species that contaminate carcasses originate in in-plant slaughterhouses and/or supply water. The present study aimed to determine the source of(More)
Since the introduction of the genus Arcobacter in 1991, the association of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii with humans and animals has been clearly established. These bacteria have been detected world wide in products of animal origin and in healthy animals as well as in surface water. A fourth species Arcobacter(More)
Campylobacter jejuni 81116 is able to switch flagellum formation on and off. To study the expression of flagellin, the main component of flagella, an expression library of C. jejuni DNA was constructed in lambda gt11. Screening of this library with a flagellin-specific antiserum resulted in a clone producing a beta-galactosidase-flagellin fusion protein; it(More)