William McFadden

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We describe a general, versatile and minimally invasive method to image single molecules near the cell surface that can be applied to any GFP-tagged protein in Caenorhabditis elegans embryos. We exploited tunable expression via RNAi and a dynamically exchanging monomer pool to achieve fast, continuous single-molecule imaging at optimal densities with(More)
Pulsed actomyosin contractility underlies diverse modes of tissue morphogenesis, but the mechanisms that generate pulsed contractions are still poorly understood. Here, we combine quantitative imaging with genetic perturbations and mathematical modeling to identify a core mechanism for pulsed contractility in early C. elegans embryos. We show that pulsed(More)
We describe a general, versatile and non-invasive method to image single molecules near the cell surface that can be applied to any GFP-tagged protein in C. elegans embryos. We exploit tunable expression via RNAi and a dynamically exchanging monomer pool to achieve fast continuous single-molecule imaging at optimal densities with signal-to-noise ratios(More)
We have designed a framework for content based appraisal of documents. Our motivation is to provide computer assisted support for answering several appraisal criteria according to the general appraisal guidelines in the National Archives and Record Administration (NARA) 1441 directive. The appraisal criteria led us to investigations related to (a) finding(More)
This work addresses the problem of designing a scalable framework for archival appraisals of contemporary PDF documents. The motivation for our work is to provide an e-science solution that (a) fuses the independent research methodologies focusing on specific information types to one comprehensive analytical framework, (b) optimizes tradeoffs between(More)
Actomyosin-based cortical flow is a fundamental engine for cellular morphogenesis. Cortical flows are generated by cross-linked networks of actin filaments and myosin motors, in which active stress produced by motor activity is opposed by passive resistance to network deformation. Continuous flow requires local remodeling through crosslink unbinding and(More)
This book chapter describes problems related to contemporary document analyses. Contemporary documents contain multiple digital objects of different type. These digital objects have to be extracted from document containers, represented as data structures, and described by features suitable for comparing digital objects. In many archival and machine learning(More)
INTRODUCTION Many neural processes, such as memory formation and learning, occur over a relatively long time frame. Conventional neural electrodes lose much of their accuracy over these time periods, due to multiple factors including glial cell encapsulation, micromovement in the brain, and neuron cell death. To address this problem, electrodes for(More)
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