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for help in performing the isozyme analysis. stumps or trees were S; isolates from pine stumps were 80% S and 20% J. 1996. Use of taxon-specific competitive-priming PCR to study host P; isolates from pine, incense cedar, and western juniper trees were 23% specificity, hybridization, and intergroup gene flow in intersterility groups S and 77% l? The recovery(More)
and F intersterility groups were subjected to isozyme analysis. European S, P, and F groups had more variability than the North American S and P groups in expected hterozygosity, number of alleles per locus, and percent polymorphic loci. In contrast with the North American S and P groups, the European intersterility groups could not be distinguished from(More)
The genus Heterobasidion includes some of the most important pathogens of conifers in the world, and as such it is one of the most intensely studied genera of fungi. Because of the remarkable paucity of distinguishing morphological traits, the taxonomy of species within this genus has always been problematic. A partial resolution of the taxonomic issues(More)
Fungi in the basidiomycete species complex Heterobasidion annosum are significant root-rot pathogens of conifers throughout the northern hemisphere. We utilize a multilocus phylogenetic approach to examine hypotheses regarding the evolution and divergence of two Heterobasidion taxa associated with pines: the Eurasian H. annosum sensu stricto and the North(More)
Heterobusidion unnosum. The isolates belonged lo the " S " and " P " intersterility groups and were collected from live conifer species found in the western United States. Ten allozyme loci distributed among eight enzyme systems were examined. Intersterility groups differed at nine loci. intersterility groups indicates an extremely high degree of genetic(More)
A 35-year-old longleaf pine stand exhibited trees in various stages of decline. A study was conducted to determine root-infecting fungi and other abnormalities associated with varying degrees of crown symptoms. A four-class crown symptom rating system was devised according to ascending symptom severity. Leptographium procerum and L. terebrantis were(More)
We determined the effects of subsoiling on woody lateral roots and enzyme activities involved in stem carbon metabolism of 90-to loo-year-old Jeffrey pine (Pinusjefreyi Grev. and Balf.) growing on the eastern side of the California Sierra Nevada Range. Twelve 1 .O-ha plots were established on each of two sites. Four site treatments-thinning and subsoil-ing(More)
Ergosterol, a membrane sterol found in fungi but not in plants, was used to estimate live mycelial biomass in ectomycorrhizae. Loblolly pine (Pinus taeda L.) seeds were sown in April 1993 and grown with standard nursery culture practices. Correlations between total seedling ergosterol and visual assessment of mycorrhizal colonization were high during July(More)
A natural first generation hybrid fungus shows interspecific heterozygosity. The nuclear condition of a rare natural hybrid between two taxa of the Heterobasidion complex is investigated. Heterobasidion species are known to be either homokaryotic (haploid) or heterokaryotic (n+n), but heterokaryons are made up of both homokaryotic and heterokaryotic(More)