William H. Cernota

Publications12
h-index7
Citations252
Highly Influential Citations8
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Engineering precursor flow for increased erythromycin production in Aeromicrobium erythreum.
Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production.
Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea
TLDR
A metabolic model is proposed where in carbohydrate-based fermentations MCM acts as a drain on the methylmalonyl-CoA metabolite pool, and in oil- based fermentations,MCM acts in the reverse direction to fill the methyl malonyl -CoA pool.
The erythromycin biosynthetic gene cluster of Aeromicrobium erythreum
TLDR
The gene order of the two ery clusters was conserved within a core region of 15 contiguous genes, with the exception of IS1136 which was not found in the A. erythreum cluster.
Analysis of an 8.1-kb DNA Fragment Contiguous with the Erythromycin Gene Cluster of Saccharopolyspora erythraea in the eryCI-Flanking Region
TLDR
These results complete the analysis of the erythromycin gene cluster and eliminate the possibility that additional sought-after pathway-specific structural or regulatory genes might be found within or adjacent to the cluster.
Knockout of the Erythromycin Biosynthetic Cluster Gene, eryBI, Blocks Isoflavone Glucoside Bioconversion during Erythromycin Fermentations in Aeromicrobium erythreum but Not in Saccharopolyspora…
TLDR
This study showed that isoflavone metabolism could be blocked in A. erythreum by ery BI knockout but that eryBI knockout was not sufficient to block is oflav one metabolism in S. eries, suggesting that other β-glucosidases are present.
Biotransformation and recovery of the isoflavones genistein and daidzein from industrial antibiotic fermentations
The objective of this study was to follow the metabolic fate of isoflavone glucosides from the soybean meal in a model industrial fermentation to determine if commercially useful isoflavones could be…
Random transposon mutagenesis of the Saccharopolyspora erythraea genome reveals additional genes influencing erythromycin biosynthesis.
TLDR
It is demonstrated that random transposon mutagenesis uncovers strain improvement-related genes potentially useful for strain engineering.
An erythromycin process improvement using the diethyl methylmalonate-responsive (Dmr) phenotype of the Saccharopolyspora erythraea mutB strain
TLDR
The Saccharopolyspora erythraea mutB knockout strain, FL2281, was found to carry a diethyl methylmalonate-responsive (Dmr) phenotype, which represents a new class of strain improvement phenotype and a theory to explain the biochemical mechanism for the Dmr phenotype is proposed.
Application of In Vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea.
TLDR
The in vitro method involves a significant investment in time and effort to create the mutants, but once the mutants are made and screened, a large number of highly relevant mutations of direct interest to erythromycin production can be found.
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