William G Bendena

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Cytological studies have shown that the heat shock loci 93D of Drosophila melanogaster and 2-48B of Drosophila hydei have several characteristics which suggest that they are homologous loci, yet sequence homology is barely detectable by cross-hybridization. Using cloned DNA sequences we have compared the two loci. Both loci produce transcripts of similar(More)
Using HPLC separation, radioimmunoassay, and subsequent bioassay, we have detected the presence of an active peptide, which co-elutes with the insect myoinhibitory peptide leuco-myosuppressin, in the brain of the cockroach Diploptera punctata. We have isolated a cDNA encoding the precursor for this peptide from cDNA libraries representing D. punctata brain(More)
A 15-residue neuropeptide, Manduca sexta allatostatin (Mas-AST), strongly inhibits juvenile hormone (JH) biosynthesis in vitro by corpora allata (CA) from Manduca fifth-stadium larvae and adult females as well as Helicoverpa zea adult females (Kramer et al., 1991 Proc. Natl. Acad. Sci (USA) 88, 9458-9462). In contrast, this study found that 1.0 microM(More)
The isoprenoid methyl farnesoate (MF) has been implicated in the regulation of crustacean development and reproduction in conjunction with eyestalk molt inhibiting hormones and ecdysteroids. Farnesoic acid O-methyltransferase (FAMeT) catalyzes the methylation of farnesoic acid (FA) to produce MF in the terminal step of MF synthesis. We have previously(More)
Juvenile hormone (JH) regulates many developmental and physiological events in insects, but its molecular mechanism remains conjectural. Here we report that genetic ablation of the corpus allatum cells of the Drosophila ring gland (the JH source) resulted in JH deficiency, pupal lethality and precocious and enhanced programmed cell death (PCD) of the larval(More)
Allatostatins are a family of peptides that inhibit the production of juvenile hormone in the cockroach, Diploptera punctata. It is likely that the allatostatin prohormone precursor is processed to give rise to all 13 members of the family simultaneously. All members of the family show potency and efficacy, in terms of their ability to inhibit juvenile(More)
The Drosophila hsr omega locus produces one of the largest and most active heat shock puffs, yet it does not encode a heat shock protein. Instead, this locus produces a distinctive set of three transcripts, all from the same start site. The largest transcript, omega 1, is limited to the nucleus and appears to have a role there. A second nuclear transcript,(More)
The hsr-omega locus forms one of the largest Drosophila heat-shock puffs and produces three major transcripts. These three transcripts are also produced constitutively, at lower levels, in almost all tissues and developmental stages. The amounts of the transcripts in nonstressed cells are modulated during development. The hormone ecdysone leads to increased(More)
Neuropeptides of the cockroach allatostatin (AST) family are known for their ability to inhibit the production of juvenile hormone by the corpora allata of cockroaches. Since their discovery, they have also been shown to modulate myotropic activity in a range of insect species as well as to act as neurotransmitters in Crustaceans and possibly in insects.(More)
Allatostatins (ASTs) are insect neuropeptides that inhibit juvenile hormone biosynthesis by the corpora allata. We have isolated a cDNA from the cockroach Diploptera punctata that encodes a 41.5-kDa precursor polypeptide containing the AST family of peptides. Translation of the cDNA revealed a 370-amino acid pre-pro-peptide consisting of 13 AST-type(More)