William F. Farnham

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Mushroom-forming fungi (homobasidiomycetes) are major examples of morphological and ecological diversification in terrestrial habitats. Homobasidiomycetes includes only nine described species that are known from marine environments. Morphological traits that have concealed the ancestry of these fungi include reduced fruiting bodies with hairy surfaces and(More)
Isolated mouse parotid acinar cells (acini) were prepared by enzyme digestion, divalent cation depletion, and mechanical shearing. Acini were found to be morphologically intact, i.e., 95% viable as judged by trypan blue exclusion. Amylase release by the cholinergic agonist carbachol, by the beta-adrenergic agonist isoproterenol, and by monensin was similar(More)
Twenty-and 22-carbon fatty acids of the linoleic (n-6) and linolenic (n-3) acid families were measured in murine spermatogonia and preleptotene spermatocytes (early), pachytene primary spermatocytes (10) round spermatids (RS), condensing spermatids (CS) and Leydig cells enriched by staput velocity sedimentation at 1 G, followed by viable microflow sorting(More)
The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells were enriched to 90%, a 10-fold purification. The capability for viable sorting of most testis cell(More)
Rat and mouse parotid acinar cells have been dispersed into small acinar groups and individual cells. Amylase release in response to secretagogues has been determined. Cytochemical localization of adenylate cyclase in these cells has demonstrated isoproterenol-stimulated adenylate cyclase in the same location as the reported in tissue slices, at the(More)
cooler conditions and stormier weather. To interpret these changes, we measured recruitment, growth and production of tetraspores at sheltered and exposed sites in 2012–2014, years which had low and high spring temperatures. Potential spore production was greater at the sheltered site due to a longer growing period and survival of larger fronds. Delayed(More)
The role of calcium ions in the activation of guanylate cyclase and in the propagation of the effects of cGMP during activation of the muscarinic cholinergic receptors was studied in isolated rat parotid acinar cells. It was demonstrated that the requirement for extracellular calcium is complete in the transduction of information from the muscarinic(More)
The morphology and composition of isolated acinar cells from the enlarged parotid glands of rats treated chronically with DL-isoproterenol were investigated and compared with isolated acinar cells from parotids of control untreated rats. Changes similar to those observed in parotid acinar cells from patients with cystic fibrosis were demonstrated.
The handling of Ca by the rat parotid gland was investigated using micropuncture, microperfusion, and microanalytical techniques. Concentrations of Ca were measured in salivary fluid from intercalated, lobular and main ducts, and net transductal fluxes of Ca and water were calculated during stimulation of secretion with acetylcholine, pilocarpine, and(More)
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