HIFα Targeted for VHL-Mediated Destruction by Proline Hydroxylation: Implications for O2 Sensing
It is found that human pVHL binds to a short HIF-derived peptide when a conserved proline residue at the core of this peptide is hydroxylated, which may play a key role in mammalian oxygen sensing.
Endostatin: An Endogenous Inhibitor of Angiogenesis and Tumor Growth
Insulin-stimulated Phosphorylation of a Rab GTPase-activating Protein Regulates GLUT4 Translocation*
Findings strongly indicate that insulin-stimulated phosphorylation of AS160 is required for GLUT4 translocation and that this phosphorylated signals translocation through inactivation of the Rab GAP function.
ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation.
BACH1, a Novel Helicase-like Protein, Interacts Directly with BRCA1 and Contributes to Its DNA Repair Function
The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins
- G. Orphanides, Wei-Hua Wu, William Arbuthnot Sir Lane, M. Hampsey, D. Reinberg
- 15 July 1999
The biochemical analysis of FACT is provided, providing evidence that Spt16/Cdc68 is involved in transcript elongation in vivo, and FACT specifically interacts with nucleosomes and histone H2A/H2B dimers, indicating that it may work by promoting nucleosome disassembly upon transcription.
A core SMRT corepressor complex containing HDAC3 and TBL1, a WD40-repeat protein linked to deafness.
- M. Guenther, William Arbuthnot Sir Lane, W. Fischle, E. Verdin, M. Lazar, R. Shiekhattar
- BiologyGenes & Development
- 1 May 2000
This work reports the isolation of a novel SMRT-containing complex from HeLa cells that contains transducin beta-like protein 1 (TBL1), whose gene is mutated in human sensorineural deafness and contains HDAC3, a histone deacetylase not previously thought to interact with SMRT.
Ordered Phosphorylation Governs Oscillation of a Three-Protein Circadian Clock
- Michael J Rust, J. Markson, William Arbuthnot Sir Lane, D. Fisher, E. O’Shea
- 2 November 2007
A mathematical model constrained by experimental data quantitatively reproduces the circadian period and the distinctive dynamics of the four phosphoforms shown in an ordered pattern arising from the intrinsic autokinase and autophosphatase rates of KaiC and their modulation by KaiA.
Acetylation of the p53 DNA-binding domain regulates apoptosis induction.
SR proteins: a conserved family of pre-mRNA splicing factors.
It is demonstrated that four different proteins from calf thymus are able to restore splicing in the same splicing-deficient extract using several different pre-mRNA substrates, and a repeated protein sequence that encompasses an RNA recognition motif was observed.