Wilfried Tillmann

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Ca2+ ions were transported into the cell by incubation of the erythrocyte suspension with ionophore A23187, a lipophil electric neutral ion complexing substance. Erythrocyte aggregation could be increased twice, when doubling the intracellular Ca2+-ion concentration. Our measurements lead to the suggestion that an increase of the cytoplasmatic Ca2+ ion(More)
Bei einem 1970 beschriebenen Patienten mit kongenitaler nichtsphärozytärer hämolytischer Anämie und seinen heterozygoten Eltern wird das defiziente Enzym Glucosephosphatisomerase (GPI) in Erythrozyten und Leukozyten näher charakterisiert. Die Enzymvariante unterscheidet sich von bekannten GPI-Varianten durch die elektrophoretische Mobilität, durch die(More)
The rheological properties of erythrocytes of four patients with haemoglobin Köln (Hb Köln) disease were studied. Filtration of erythrocytes through polycarbonate sieves with a pore diameter of 5 mum and viscosity measurements of erythrocytes suspensions with a PCV of 80% were carried out. The rheological properties of erythrocytes of two of the patients(More)
The rheological properties of erythrocytes were studied in 12 heterozygous and nine homozygous beta thalassaemia patients, using filtration of erythrocytes through polycarbonate sieves with a pore diameter of 5 micron and viscosity measurements of erythrocyte and ghost suspensions. Erythrocyte deformability of all patients was decreased. In the heterozygous(More)
Die Pyruvatkinaseaktivität von Erythrozytenmembranen, die normalerweise maskiert ist, wurde nach mechanischer Zerstörung der Membranen bei normalen Personen und bei drei homozygoten Patienten mit Pyruvatkinasemangel bestimmt. Obgleich die Patienten 1 und 2, die Geschwister sind, relativ hohe Enzymaktivitäten in ihren Hämolysaten haben, litten sie unter der(More)
Deformability of erythrocytes from four patients with different types of glucosephosphate isomerase (D-glucose-6-phosphate ketoisomerase, GPI) deficiency has been determined by cell filtration. Young as well as whole erythrocyte populations had a markedly increased rigidity and an abnormally strong attachment of haemoglobin to the inner surface of isolated(More)
Double heterozygosity of pyruvate kinase (PK) deficiency associated with hereditary hemolytic anemia is emphasized by studies of a kindred harboring two distinct mutant forms of this enzyme. The hematologically unaffected parents exhibit slightly reduced PK activity, a normal Hill coefficient, and a normal thermodynamic dissociation constant for the overall(More)
The influence of temperature, K+, Mg2+ and fructose 1,6-bisphosphate on human red cell pyruvate kinase was investigated. Kinetic measurements between 4 degrees C and 43 degrees C revealed a remarkable influence of the temperature on the allosteric behaviour of the enzyme. Below a transition region between 15 degrees C and 20 degrees C (as obtained from an(More)
Bisherige Untersuchungen über die Beziehungen zwischen enzymatisch-katalysierter ATP-Bildung in der Erythrozytenmembran und dem Natrium- und Kaliumtransport haben widersprüchliche Ergebnisse gezeigt. Die vorliegende Arbeit zeigt, daß die Membran menschlicher Erythrozyten eine nicht unbedeutende Menge Pyruvatkinase enthält. Membranbruchstücke bilden aus(More)