Wiggert A. van Cappellen

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Cell segmentation and tracking in time-lapse fluorescence microscopy images is a task of fundamental importance in many biological studies on cell migration and proliferation. In recent years, level sets have been shown to provide a very appropriate framework for this purpose, as they are well suited to capture topological changes occurring during mitosis,(More)
A remarkable and yet unexplained phenomenon in cancer cells is the presence of multiple centrosomes, organelles required for normal cell division. Previously, it was demonstrated that the tumor suppressor BRCA1 is a component of centrosomes. This observation led to the hypothesis that defective BRCA1 results in malfunctioning centrosomes and faulty(More)
To improve nanoliposomal-doxorubicin (DoxNL) delivery in tumor cells using liposome membrane-incorporated short-chain sphingolipids (SCS) with selective membrane-permeabilizing properties. DoxNL bilayers contained synthetic short-chain derivatives of known membrane microdomain-forming sphingolipids; C8-glucosylceramide (C8-GluCer), C8-galactosylceramide(More)
MOTIVATION Complete, accurate and reproducible analysis of intracellular foci from fluorescence microscopy image sequences of live cells requires full automation of all processing steps involved: cell segmentation and tracking followed by foci segmentation and pattern analysis. Integrated systems for this purpose are lacking. RESULTS Extending our(More)
Time-lapse confocal microscopy of mouse embryo slices was developed to access and image the living aorta. In this paper, we explain how to label all hematopoietic and endothelial cells inside the intact mouse aorta with fluorescent directly labeled antibodies. Then we describe the technique to cut nonfixed labeled embryos into thick slices that are further(More)
Developments in radio astronomy instrumentation drive the need for lower cost front-ends due to the large number of antennas and low noise amplifiers needed. This paper describes cost reduction techniques for the realization of antennas and low noise amplifiers in combination with a noise budget calculation for array systems in the absence of cryogenic(More)
The ultimate aim of many live-cell fluorescence microscopy imaging experiments is the quantitative analysis of the spatial structure and temporal behavior of intracellular objects. This requires finding the precise geometrical correspondence between the time frames for each individual cell and performing intracellular segmentation. In a previous paper we(More)
The Rockefeller University Press $30.00 J. Cell Biol. Vol. 207 No. 5 599–613 www.jcb.org/cgi/doi/10.1083/jcb.201405014 JCB 599 *M. Reuter and A. Zelensky contributed equally to this paper. Correspondence to Claire Wyman: c.wyman@erasmusmc.nl Abbreviations used in this paper: ACF, autocorrelation function; CDF, cumulative distribution function; DSB,(More)
Cassiopeia A was observed using the low-band antennas of the LOw Frequency ARray (LOFAR) with high spectral resolution. This allowed a search for radio recombination lines (RRLs) along the line-of-sight to this source. Five carbon α RRLs were detected in absorption between 40 and 50 MHz with a signal-to-noise ratio of >5 from two independent LOFAR data(More)
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