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In biological tissues, specific carbohydrate moieties of the oligosaccharide chains of glycoproteins can be localized by lectin binding. Such carbohydrate moieties are among the factors that mediate cell-cell or cell-matrix interactions during pre- and postimplantation embryonic development. Binding sites for the lectins RCA I, WGA, and LTA were localized(More)
An in situ embedding technique for monolayer cultures for subsequent ultrastructural studies is described. Dehydration is carried out in graded ethanol series without propylene oxide, and Epon is used as a final embedding medium. The advantage of this method is that the plastic flask with the embedded material can be stripped off easily without affecting(More)
Growth cones of differentiated neuroblastoma cells in monolayer cultures were studied by electron microscopy. Morphological differentiation of the growth cone formation was induced by sodium bromide. Upon prolonged application of 10(-4) to 10(-5) M sodium bromide to the cultures, a peculiar or modified formation of the growth cone occurred. Growth cones(More)
Neuroblastoma cells grown on substrates in culture develop long processes and assume the morphology of normal neurons as judged light microscopically. The development of synapses in the cultured tissue is studied by periodic electron microscopic examination of the areas of contact between cells. The initial expiants are free of any apparent synaptic(More)
Cultured murine neuroblastoma cells contain an extensive smooth endoplasmic reticulum (SER), also within their numerous processes and their terminals, consisting of a complex network of sacs and tubules. Both the perikaryal and the SER found within the processes and endings resemble one another morphologically. From the SER whether it is within the(More)
Wireless communication demands for specialized protocols secure against attacks on the radio path while fitting the limited calculation and memory capabilities of mobile terminals. To ensure accessibility of mobile services beyond a user's home network, signing on a foreign network should be possible. The latter must be able to authenticate a user without(More)
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