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Symbiotic germination was analyzed in the myco-heterotrophic orchid Gastrodia elata. Seeds were placed on water agar medium containing a leaf-disc of Quercus accutissima that had been previously inoculated with one of six different Mycena species. Among the six fungi, KFRI1212 (HQ662845) and KFRI2121 (HQ662846) germinated 60.1 and 47.0 % seeds,(More)
Gastrodia elata must establish symbiotic associations with different compatible fungi at seed germination and during vegetative growth. One of the major problems associated with its field production is the degeneration of tubers, which is mainly caused by multiple regeneration via vegetative propagation using immature tubers. To overcome this problem, an in(More)
Gastrodia elata is a myco-heterotrophic orchid that has been used in traditional medicine to treat number of human illnesses. It establishes symbiotic associations with compatible mycorrhizal fungi, such as Mycena species and Armillaria mellea, depending on its developmental stages. G. elata tubers contain two major pharmacological substances; gastrodin(More)
Ergone,a fungal metabolite derived from ergosterol,was previously isolated and identified from Polyporus umbellatus. Ergone is a major component of P. umbellatus known to have anti-aldosteronic diuretic effect and also displays cytotoxic activities. Most of mushroom's fruit bodies used for test contained less than 10 µg/g of ergone. But P. umbellatus have(More)
The levels of ergothioneine (ERG), which have been shown to act as an excellent antioxidant, were determined in both fruiting bodies and mycelia of various mushroom species. We found that ERG accumulated at different levels in fruiting bodies of mushrooms and showed up to a 92.3-fold difference between mushrooms. We also found that ERG accumulated at higher(More)
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