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Establishing an infection within a host requires efficient invasion and division. In the intracellular parasite Toxoplasma gondii, these functions are mediated by dynamin-related proteins with a unique evolutionary history.
Over-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schizonts produces a punctate pattern of fluorescence typical of rhoptries, secretory organelles involved in host cell invasion. The GFP-positive bodies were purified by a combination of differential and density gradient centrifugation and their protein content determined by MS/MS… (More)