Wesler Lambert

Learn More
The purpose of the present study was to establish a rat retinal ganglion cell line by transformation of rat retinal cells. For this investigation, retinal cells were isolated from postnatal day 1 (PN1) rats and transformed with the psi2 E1A virus. In order to isolate retinal ganglion cells (RGC), single cell clones were chosen at random from the transformed(More)
PURPOSE To determine whether cells and tissue from the human lamina cribrosa (LC) express neurotrophin and tyrosine kinase (trk) receptor mRNA and protein and whether these cells secrete neurotrophins. METHODS Synthesis of cDNA and the reverse transcription-polymerase chain reaction (RT-PCR) were conducted using total RNA obtained from well-characterized(More)
PURPOSE To compare the mRNA expression of growth factor receptors in cultured human trabecular meshwork (HTM) cells with ex vivo HTM tissues and to determine whether HTM cells generate a physiologic response after exposure to exogenous growth factors. METHODS The reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the(More)
Paul Farmer*, Charles Patrick Almazor, Emily T. Bahnsen, Donna Barry, Junior Bazile, Barry R. Bloom, Niranjan Bose, Thomas Brewer, Stephen B. Calderwood, John D. Clemens, Alejandro Cravioto, Eddy Eustache, Gregory Jérôme, Neha Gupta, Jason B. Harris, Howard H. Hiatt, Cassia Holstein, Peter J. Hotez, Louise C. Ivers, Vanessa B. Kerry, Serena P. Koenig,(More)
PURPOSE Growth factors act through high-affinity cell surface receptors expressed by target cells and are critical modulators of cell function. Because aqueous humor is known to contain growth factors, these molecules may play a key role in maintaining the normal function of the human trabecular meshwork (HTM). Alternate mRNA splicing is an important(More)
Three decades ago, the world's ministries of health declared primary health care--the delivery of basic preventive and curative services--a top priority. Since then, however, the world's poorest countries have not met most primary health care goals. Twenty-six years after the Declaration of Alma Ata, we are said to be living in a time of "limited(More)
PURPOSE The purpose of this study was to compare the mRNA expression of neurotrophins (NTs) and NT receptors (Trk) in cultured human trabecular meshwork (HTM) cells and ex vivo HTM tissues, to immunolocalize both NT and Trk receptors in cultured HTM cells, and to demonstrate secretion of NTs by HTM cells. METHODS Reverse transcription-polymerase chain(More)
INTRODUCTION At least 36 countries are suffering from severe shortages of healthcare workers and this crisis of human resources in developing countries is a major obstacle to scale-up of HIV care. We performed a case study to evaluate a health service delivery model where a task-shifting approach to HIV care had been undertaken with tasks shifted from(More)
Access to antiretroviral treatment and care : experience of the HIV Equity Initiative, Cange, Haiti : case study. (Perspectives and practice in antiretroviral treatment) 1.HIV infection – drug therapy 2. HIV infection-prevention and control 3. Acquired immunodeficiency syndrome 4.Antiretroviral therapy, Highly active-utilization 5.Health services(More)
In many settings worldwide, HIV-positive individuals have experienced a significant level of stigma and discrimination. This discrimination may also impact other family members affected by the disease, including children. The aim of our study was to identify factors associated with stigma and/or discrimination among HIV-affected youth and their HIV-positive(More)