Werner Grüning

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Two distinct signaling pathways, involving Wnt signaling and polycystin, have been found to be critical for normal kidney development. Renal tubulogenesis requires the presence of certain Wnt proteins, whereas mutations in polycystin impede the terminal differentiation of renal tubular epithelial cells, causing the development of large cystic kidneys that(More)
Autosomal dominant polycystic kidney disease (ADPKD) is a common hereditary disorder that accounts for 8-10% of end stage renal disease. PKD1, one of two recently isolated ADPKD gene products, has been implicated in cell-cell and cell-matrix interactions. However, the signaling pathway of PKD1 remains undefined. We found that the C-terminal 226 amino acids(More)
The Wnt-inducible homeobox gene Siamois is expressed in Xenopus embryos before gastrulation and is necessary for formation of the Spemann organizer. Here we show that 5'-flanking sequences of the Siamois coding region can specifically activate a heterologous reporter gene in dorsovegetal cells, thus mimicking Siamois's endogenous expression. A 245-bp DNA(More)
Somatic mutations of the receptor tyrosine kinase Flt3 consisting of internal tandem duplications (ITD) occur in 20% of patients with acute myeloid leukemia. They are associated with a poor prognosis of the disease. In this study, we characterized the oncogenic potential and signaling properties of Flt3 mutations. We constructed chimeric molecules that(More)
The von Hippel-Lindau (VHL) tumor suppressor gene is mutated in patients with VHL disease and in the majority of patients with sporadic clear cell renal carcinoma (RCC). Overexpression of transforming growth factor (TGF) beta1 has been observed in patients with several cancers, including RCCs, with serum and urine levels correlating inversely with(More)
The recently discovered family of regulators of G protein signaling (RGS) accelerates the intrinsic GTPase activity of certain Galpha subunits, thereby terminating G protein signaling. Particularly high mRNA levels of one family member, RGS3, are found in the adult kidney. To establish the temporal and spatial renal expression pattern of RGS3, a polyclonal(More)
1. Es wird der Nachweis erbracht, daß die Antithrombinwirkung des Plasmaalbumins nur an eine bestimmte Albuminfraktion gebunden ist. Diese wird durch 45% Ammonsulfat zur Ausfällung gebracht. Durch Alterung geht der gerinnungshemmende Einfluß jener Fraktion all-mählich zurück und verschwindet nach etwa 50 Tagen vollkommen. 2. Die Angaben vonWöhlisch(More)
Es wird eine Bestimmungsmethode der sauren Serumphosphatase beschrieben, die Natrium-β-Glycerophosphat als Substrat verwendet. Ihre routinemäßige, klinische Anwendung hat die Brauchbarkeit und Zuverlässigkeit dieser Analyse erwiesen. Bei normalen Erwachsenen liegt die durchschnittliche Serumphosphatasektivität bei 1,2±0,4 PE. Bei Kranken mit(More)