Werner Edgar Gläßgen

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Six anthocyanins were isolated from cell suspension cultures of an Afghan cultivar of Daucus carota by PC or HPLC. The structures of these compounds were elucidated by spectroscopic methods as cyanidin 3-O-lathyroside, cyanidin 3-O-(2''-O-beta-D-xylopyranosyl-6''-O-beta-D-glucopyranosyl-beta-D- galactopyranoside), and the latter acylated with 4-coumaric,(More)
A postcolumn receptor-affinity detection (RAD) was developed for the detection of urokinase and cross-reactive compounds. The analytical method consisted of gradient reversed-phase HPLC coupled on-line to a RAD system based on fluorescein-labelled urokinase receptor (fluorescein-uPAR) as reagent. Fluorescein-uPAR was added continuously to the HPLC effluent(More)
The accumulation of anthocyanins in cell cultures of Daucus carota L. and the enzymes involved in their biosynthesis were investigated under growth in the dark, continuous irradiation with UV light, incubation with elicitors from Pythium aphanidermatum, and elicitor treatment of UV-irradiated cells. Upon UV irradiation, anthocyanin accumulation was strongly(More)
Protein preparations from cell-suspension cultures of an Afghan cultivar of Daucus carota (carrot) catalyzed the formation of acylated anthocyanins from a cyanidin triglycoside isolated from the carrot cultures using (1-O-sinapoyl-, 1-O-feruloyl-) and 1-O-(p-coumaroyl)-β-glucose as acyl donors. The enzyme activities can be classified as(More)
Anthocyanins isolated and characterized from the wild carrot suspension cultures used here were 3-O-β-D-glucopyranosyl-(1→6)-[β-D-xylopyranosyl-(1→2)-]β-D<-galactopyranosylcyanidin (1), 3-O-[β-D- xylopyranosyl-(1→2)-β-D-galactopyranosyl]cyanidin (2), 3-O-(6-O-sinapoyl)-β-D-glucopyranosyl-(1→6)-[β-D- xylopyranosyl-(1→2)-]β-D-galactopyranos ylcyanidin (3),(More)
Callus cell lines of potato (Solanum tuberosum L. cv. Zarevo) were obtained from seedlings germinated from gamma-irradiated seeds (200 Gy). Some of these cell lines produce red-violet pigments which were identified as acylated anthocyanins. The major anthocyanin was determined to be peonidin 3-O-[6-O-(4-O-E-p-coumaroyl-rhamnosyl)-glucoside]-5-O-glucoside(More)
The major anthocyanins accumulated by an Afghan cultivar ofDaucus carota L. are cyanidin 3-(xylosylglucosylgalactosides) acylated with sinapic or ferulic acid. The formation of the branched triglycoside present as a common structural element requires an ordered sequence of glycosylation events. Two of these enzymic glycosylation reactions have been detected(More)
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