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OBJECTIVE To investigate the effect of knocking-down microRNA-221 (miR-221) expression on the radiosensitivity of human colorectal carcinoma cells. METHODS Human colorectal carcinoma-derived cell line Caco2 was transfected with miR-221 antisense oligonucleotides (anti-miR-221) via Lipofectamine 2000. Real-time quantitative PCR was performed to detect the(More)
Efficient emergency rehearse system provides both commanders and evacuation crowds with better preparation for dangerous gas diffusion accidents, which definitely causes serious social panic and huge property loss. This paper focuses on four key issues raised by such accidents in the chemical industry parks. These issues include modeling, simulation,(More)
OBJECTIVE To investigate the characterization of intestinal epithelial stem cells stained by Rhodamine 123 (Rho) and analyze the dynamic changes of intestinal epithelial stem cells during the injured-repaired progress induced by 5-FU. METHODS Mucosal cells were obtained from adult C57BL/6J mice. The Rho stained cells were sorted using FACS. The mouse(More)
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