Wenfeng Zhong

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We present a multi-functional electroporation method for delivery of biomolecule utilizing a high-density distributed electrode network (HDEN) under tri-phase electric stimulation. The HDEN device, with which drastic pH change during the electroporation was avoided,was demonstrated to be highly effective for transfection of not only DNA plasmids and small(More)
We have developed a rapid method to optimize the electric parameters of cell electroporation. In our design, a pair of ring-dot formatted electrodes was used to generate a radial distribution of electric field from the center to the periphery. Varied electric field intensity was acquired in different annulus when an electric pulse was applied. Cells were(More)
In the present study, the polycyclic aromatic hydrocarbons (PAHs) in indoor dust and outdoor dust including road and window dust around the traffic road in Hunan Province, China, were sampled and detected. The ∑PAHs in indoor dust ranged from 5007-24,236 ng g(-1), with a median of 14,049 ng g(-1). The ∑PAHs in road dust ranged from 3644-12,875 ng g(-1),(More)
Electroporation has been widely used in delivering foreign biomolecules into cells, but there is still much room for improvement, such as cell viability and integrity. In this manuscript, we investigate the distribution and the toxicity of pH changes during electroporation, which significantly decreases cell viability. A localized pH gradient forms between(More)
Continuous cell electroporation is an appealing non-viral approach for genetically transfecting a large number of cells. Yet the traditional macro-scale devices suffer from the unsatisfactory transfection efficiency and/or cell viability due to their high voltage, while the emerging microfluidic electroporation devices is still limited by their low cell(More)
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