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Understanding ligand binding effects on the conformation of estrogen receptor alpha-DNA complexes: a combinational quartz crystal microbalance with dissipation and surface plasmon resonance study.
- Wendy Y X Peh, E. Reimhult, Huey Fang Teh, J. Thomsen, X. Su
- Medicine, Chemistry
- Biophysical journal
- 15 June 2007
Estrogen receptors are ligand-activated transcription factors that regulate gene expression by binding to specific DNA sequences. To date, the effect of ligands on the conformation of estrogen… Expand
Characterization of protein--DNA interactions using surface plasmon resonance spectroscopy with various assay schemes.
Specific protein-DNA interactions play a central role in transcription and other biological processes. A comprehensive characterization of protein-DNA interactions should include information about… Expand
Combinational application of surface plasmon resonance spectroscopy and quartz crystal microbalance for studying nuclear hormone receptor-response element interactions.
- X. Su, C. Lin, S. O'Shea, Huey Fang Teh, Wendy Y X Peh, J. Thomsen
- Chemistry, Medicine
- Analytical chemistry
- 1 August 2006
Conventional methodologies for studying protein-DNA complexes, such as electrophoretic mobility shift assays (EMSAs), lack the real-time sensitivity and precision to accurately characterize the… Expand
A two-step antibody strategy for surface plasmon resonance spectroscopy detection of protein-DNA interactions in nuclear extracts.
Surface plasmon resonance (SPR) spectroscopy has emerged as a powerful alternative to conventional biochemistry methods for studying protein-DNA interactions that involve recombinant proteins of… Expand