Learn More
Phytic acid (PA) is poorly digested by humans and monogastric animals and negatively affects human/animal nutrition and the environment. Rice mutants with reduced PA content have been developed but are often associated with reduced seed weight and viability, lacking breeding value. In the present study, a new approach was explored to reduce seed PA while(More)
揭示转基因水稻全基因组遗传变异的特征与频率。 通过单核苷酸分辨率揭示了农杆菌介导法转化水 稻植株全基因组水平遗传变异的类型和频率以 及外源DNA 的整合模式。 应用Illumina Hiseq2000 高通量测序技术测定了 5 个T0 代转基因水稻株系的基因组序列。结合生 物信息学分析和聚合酶链反应(PCR)扩增, 以 及Sanger 测序, 我们检测和验证单核苷酸多态 性(SNP)和Indel 变化类型和数量, 转移DNA (T-DNA) 及其载体骨架序列和转座子整合位点 及特征, 大片段的结构变异等遗传变异。 结果表明, 农杆菌介导的水稻遗传转化, 除T-DNA 整合到水稻基因组外, 还存在载体骨架整合现 象; 每个转基因水稻基因组的变异(SNP 和小片 段的缺失插入)数目为338–1774, 与报道的组(More)
The OsLpa1 gene (LOC_Os02g57400) was identified to be involved in phytic acid (PA) metabolism because its knockout and missense mutants reduce PA content in rice grain. However, little is known about the molecular characteristics of OsLpa1 in rice and of its homologues in other plants. In the present study, the spatial pattern of OsLpa1 expression was(More)
To produce agronomically competitive rice with nutritionally superior, environmentally safe phytic acid (PA) levels, hairpin RNA (hpRNA)- and artificial microRNA (amiRNA)-mediated gene silencing approaches were explored to reduce both myo-inositol kinase gene (OsMIK) expression and PA accumulation in rice seeds. hpRNA and amiRNA sequences targeted to OsMIK(More)
  • 1