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Little is known about how foreign DNA is randomly integrated into chromosomes in transgenic animals. In the current study, the insertion sites of 36 transgenic mice were mapped by thermal asymmetric interlaced PCR, and 38 junction sequences were obtained from 30 samples. Analysis of the 38 sequences revealed that 44.7 % of integration events occurred within(More)
The aim of this study was to clone the heart-type fatty acid binding protein (H-FABP) gene of Xuhuai goat, to explore it bioinformatically, and analyze the subcellular localization using enhanced green fluorescent protein (EGFP). The results showed that the coding sequence (CDS) length of Xuhuai goat H-FABP gene was 402 bp, encoding 133 amino acids (GenBank(More)
The morphology and function of Leydig cells are changed during the development, mature and senility of Leydig cells along the life span of males. This study was to observe the growth morphology of adult mouse Leydig cells in culture, aiming to provide a reference for furthermore understanding of the biological function of Leydig cells by in vitro model.(More)
It has been demonstrated that mature cells could switch their fate by the technologies for reprogramming, such as somatic cell nuclear transfer and induced pluripotent stem cells. Recently, this conclusion was further confirmed. It was found that mature differentiated cells could be directly converted into other somatic cells or progenitors with some(More)
The process of germline development carries genetic information and preparatory totipotency across generations. The last decade has witnessed remarkable successes in the generation of germline cells from mouse pluripotent stem cells, especially induced germline cells with the capacity for producing viable offspring, suggesting clinical applications of(More)
SDD17, a delta-15 desaturase from the fungus Saprolegnia can convert arachidonic acid to eicosapentanoic acid in yeast, plant embryos, and mammalian cells. Here, we generated transgenic mice that carried two copies of codon-optimized sdd17 cDNA within a non-coding domain of chromosome 6. RT-PCR analysis revealed that the foreign gene was expressed in the(More)
Fecundity trait in sheep is regulated by some major genes. Among them, BMPR-IB, BMP-15, and GDF-9 are most distinguishing. The mutant FecB of BMPR-IB has multiplicative effects on ovulation. GDF-9's mutants FecGH, FecI, and BMP-15's mutants FecXI, FecXH, FecXG, FecXB, FecXL, and FecXR increase ovulation rate in the heterozygote but result in sterile(More)
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