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Increasing evidence has indicated that plenty of lncRNAs play important roles in many critical biological processes. Developing powerful computational models to construct lncRNA functional similarity network based on heterogeneous biological datasets is one of the most important and popular topics in the fields of both lncRNAs and complex diseases.(More)
Non-alcoholic fatty liver disease (NAFLD) has emerged as a common public health problem that can progress to end-stage liver disease. Although the function of aquaglyceroporin-9 (AQP9) as a glycerol channel has been proven, the mechanism of AQP9 in the development of NAFLD is poorly understood. To investigate the effect of hepatic glycerol uptake(More)
The aim of the present study was to employ RNA interference (RNAi) technology to construct and select shRNA-Nanog recombinant plasmids for the inhibition of Nanog gene expression and transfer these plasmids into the human gastric cancer cell line, SGC-7901, as well as to detect the expression of Nanog and the effects on the proliferation, migration,(More)
About thirty thousands horses were affected and hundreds of them died in an epidemic caused by equine 2 influenza virus (H3N8) in China. The estimated morbidity and mortality accounted for 81% and 2%, respectively. The viral protein and RNA electrophoresis patterns revealed that the new isolates were antigenically different from the prototype strain(More)
OBJECTIVE To detect large genomic deletions or duplications of ornithine transcarbamylase (OTC) gene by multiplex ligation-dependent probe amplification (MLPA). METHOD Thirty cases of suspected OTC deficiency (OTCD) patients based on tandem-mass spectrum results were recruited in Xinhua Hospital from 2012 to 2014, among whom 13 were male and 17 were(More)
AIM To investigate the effect of decorin (DCN) gene transfection on the growth of nude mice xenograft bearing human gastric carcinoma cell SGC-7901 and study the possible mechanism. METHODS Gastric carcinoma xenograft model was established in nude mice. Recombinant plasmid pEGFP-N1-DCN was injected to the center and bottom of the carcinoma; meanwhile, (More)
AIM To investigate the effect of eukaryotic expression vector pEGFP-N1-NPRL2 on the proliferation, cell cycle, apoptosis, invasion and migration of human gastric carcinoma SGC-7901 cells in vitro. METHODS The eukaryotic expression vector pEGFP-N1-NPRL2 was constructed and confirmed by enzyme digestion and sequencing analysis. Then, it was transfected(More)
Renal interstitial fibrosis (RIF) is a pathological change common to a variety of chronic renal diseases, ultimately progressing to end-stage renal failure. It is believed that epithelial cell phenotype inversion plays an important role in RIF, which is characterized by expression of the mesenchymal maker α-SMA, loss of the epithelial maker E-cadherin, and(More)
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