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Huanglongbing (HLB) or "citrus greening" is the most destructive citrus disease worldwide. In this work, we studied host responses of citrus to infection with Candidatus Liberibacter asiaticus (CaLas) using next-generation sequencing technologies. A deep mRNA profile was obtained from peel of healthy and HLB-affected fruit. It was followed by pathway and(More)
Next-generation sequencing was exploited to gain deeper insight into the response to infection by Candidatus liberibacter asiaticus (CaLas), especially the immune disregulation and metabolic dysfunction caused by source-sink disruption. Previous fruit transcriptome data were compared with additional RNA-Seq data in three tissues: immature fruit, and young(More)
OBJECTIVE This paper introduces a modified artificial immune system (AIS)-based pattern recognition method to enhance the recognition ability of the existing conventional AIS-based classification approach and demonstrates the superiority of the proposed new AIS-based method via two case studies of breast cancer diagnosis. METHODS AND MATERIALS(More)
Volatile organic compounds (VOCs) are emitted from all plants and these VOCs are important means of communication between plants and insects. It has been documented that pathogen infections alter VOC profiles rendering infected plants more attractive to specific vectors transmitting these pathogens than uninfected plants, thus potentially aiding in pathogen(More)
Cluster analysis of aerosol time-of-flight mass spectrometry (ATOFMS) data has been an effective tool for the identification of possible sources of ambient aerosols. In this study, the clustering results of two typical methods, adaptive resonance theory-based neural networks-2a (ART-2a) and density-based clustering of application with noise (DBSCAN), on(More)
Citrus tristeza virus (CTV) (genus Closterovirus) is a plant pathogen which infects economically important citrus crops such as sweet oranges, mandarins, limes and grapefruit varietals. Within the last 70 years, an estimated 100 million citrus trees have been destroyed due to CTV infection worldwide. Present measures to contain CTV infection include scouts(More)
The aerosol time-of-flight mass spectrometry (ATOFMS) has not generally been used to provide a quantitative estimation of chemical compositions of ambient aerosols. In an initial study, the possibility of developing a calibration model to predict chemical compositions from ATOFMS data was demonstrated, but because of the limited number of samples (only 12),(More)
Sensors have been widely used for disease diagnosis, environmental quality monitoring, food quality control, industrial process analysis and control, and other related fields. As a key tool for sensor data analysis, machine learning is becoming a core part of novel sensor design. Dividing a complete machine learning process into three steps: data(More)