Weishou Shen

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The effects of mineral fertilizer (NPK) and organic manure on phospholipid fatty acid profiles and microbial functional diversity were investigated in a long-term (21-year) fertilizer experiment. The experiment included nine treatments: organic manure (OM), organic manure plus fertilizer NPK (OM + NPK), fertilizer NPK (NPK), fertilizer NP (NP), fertilizer(More)
An improved bacterial preculture protocol for Agrobacterium-mediated genetic transformation was developed for an economic tomato cultivar (Solanum lycopersicum L. cv. Zhongshu No. 4). Frequencies of transient gene expression and stable transformation were influenced by the density of Agrobacterium preculture and not the density of Agrobacterium used for(More)
By generating and examining transgenic tomato overexpressing ath-miR399d grown in hydroponic conditions, in quartz sand, or in a polytunnel greenhouse vegetable soil culture, this study aimed to investigate the effects of miR399d from Arabidopsis on phosphorus (P) accumulation, P concentrations in transgenic tomato overexpressing ath-miR399d shoots,(More)
Little is known about effects of high nitrogen (N) fertilization on soil chemical properties, enzyme and microbial activities involved in N and C transformation. Soil EC, pH, mineral N, three enzyme activities, nitrification capacity, and microbial functional diversity were evaluated under conventional N rate (270 or 300 kg  N  ha−1 season−1, N4), 80% (N3),(More)
Intensive insecticide and nutrient management have been attempted worldwide to reduce citrus huanglongbing (HLB) symptom development and yield loss. However, effects of insecticide and nutrient applications on HLB have been poorly understood. Leaf nutrients, jasmonic and salicylic acid contents, cycle threshold (Ct) values of Ca. Liberibacter asiaticus(More)
The extent of soil microbial diversity in agricultural soils is critical to the maintenance of soil health and quality. The aim of this study was to investigate the influence of land use intensification on soil microbial diversity and thus the level of soil suppressiveness of cucumber Fusarium wilt. We examined three typical microbial populations, Bacillus(More)
Pelleted poultry manure is recommended for use with agricultural soil as a replacement for chemical fertilizers; however, application of the manure stimulates nitrous oxide (N2O) emission from the soil through denitrification. To mitigate the N2O emission caused by application of pelleted poultry manure, soil microcosms were set up; each microcosm was(More)
The eastern Himalayas, especially the Yarlung Zangbo Grand Canyon Nature Reserve (YNR), is a global hotspot of biodiversity because of a wide variety of climatic conditions and elevations ranging from 500 to > 7000 m above sea level (a.s.l.). The mountain ecosystems at different elevations are vulnerable to climate change; however, there has been little(More)
Soybean root rot, caused by Rhizoctonia solani, is one of the most serious soybean diseases in the North Central Region of the United States. There is no report of the relationship between Rhizoctonia root rot and soil physical and chemical properties, or microbial and nematode communities. A commercial soybean field with a long history of Rhizoctonia root(More)
The effect of transgenic insect-resistant crops on soil microorganisms has become an issue of public concern. The goal of this study was to firstly realize the variation of in situ methane (CH4) emission flux and methanogenic and methanotrophic communities due to planting transgenic Bt rice (Bt) cultivar. CH4 emitted from paddy soil was collected by static(More)