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Invasion of tumor cells into the surrounding connective tissue and blood vessels is a key step in the metastatic spread of breast tumors. Although the presence of macrophages in primary tumors is associated with increased metastatic potential, the mechanistic basis for this observation is unknown. Using a chemotaxis-based in vivo invasion assay and(More)
We have developed animal models of breast cancer that allow the direct examination of the behavior of individual green fluorescent protein-expressing carcinoma cells in live nonmetastatic and metastatic primary tumors in situ. We have combined this model with multiphoton microscopy to image differences in cell behavior within the primary tumor. Differences(More)
Understanding the mechanisms controlling cancer cell invasion and metastasis constitutes a fundamental step in setting new strategies for diagnosis, prognosis, and therapy of metastatic cancers. LIM kinase1 (LIMK1) is a member of a novel class of serine-threonine protein kinases. Cofilin, a LIMK1 substrate, is essential for the regulation of actin(More)
Access control needs to be more flexible and fine-grained to support cooperative tasks and processes performed by dynamic teams. This can be done by applying state-of-the-art role-based access control (RBAC) technology. This paper examines how to integrate RBAC in a team-based organization context and how to apply such access control to hypermedia(More)
Tailoring groupware has to deal with adapting properties of a shared information space as well as with adapting properties of the cooperation support to the group' s needs. In this paper, an approach for tailoring both aspects of groupware in an integrated fashion is proposed. This approach uses cooperative hypermedia as a unifying representation of shared(More)
We have investigated the effects of inhibiting the expression of cofilin to understand its role in protrusion dynamics in metastatic tumor cells, in particular. We show that the suppression of cofilin expression in MTLn3 cells (an apolar randomly moving amoeboid metastatic tumor cell) caused them to extend protrusions from only one pole, elongate, and move(More)
The Web has been widely used as a platform for asynchronous collaboration. However, Web based synchronous groupware are still rare, especially those using standard Web browser as front-end. From a technical and social protocol co-evolution perspective, this work examines the Web technical protocol evolution and proposes an AJAX based approach to creating(More)
We subjected cells collected using an in vivo invasion assay to cDNA microarray analysis to identify the gene expression profile of invasive carcinoma cells in primary mammary tumors. Expression of genes involved in cell division, survival, and cell motility were most dramatically changed in invasive cells indicating a population that is neither dividing(More)
We examined the role of the actin nucleation promoters neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE2 in cell protrusion in response to epidermal growth factor (EGF), a key regulator in carcinoma cell invasion. We found that WAVE2 knockdown (KD) suppresses lamellipod formation and increases filopod formation, whereas N-WASP KD has no effect.(More)