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Agrobacterium tumefaciens-mediated transformation (ATMT) is becoming a popular effective system as an insertional mutagenesis tool in filamentous fungi. To gain more insight into the cellular and molecular mechanisms in the pathogenesis of Ustilaginoidea virens, the causal agent of rice false smut disease, a T-DNA insertion mutant library of U. virens was(More)
A novel thermostable mannanase from a newly isolated Bacillus pumilus GBSW19 has been identified, expressed, purified and characterized. The enzyme shows a structure comprising a 28 amino acid signal peptide, a glycoside hydrolase family 5 (GH5) catalytic domain and no carbohydrate-binding module. The recombinant mannanase has molecular weight of 45 kDa(More)
Recently, a growing number of researches have focused on the issues raised by the knowledge discovery of online information, particularly the problems of tracking topics, ideas, and users’ spreading influence across the Web. In this paper, the search-engine query logs on Topic Detection and Tracking (TDT) is analyzed other than study of the quality of(More)
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