Wei-dong Zhao

Learn More
Membrane fusion and fission are vital for eukaryotic life. For three decades, it has been proposed that fusion is mediated by fusion between the proximal leaflets of two bilayers (hemi-fusion) to produce a hemi-fused structure, followed by fusion between the distal leaflets, whereas fission is via hemi-fission, which also produces a hemi-fused structure,(More)
Porcine β defensin 2(pBD2)is a cationic 37-amino acid antimicrobial peptide with disulfide bonds. Synthetic pBD2 had broad antimicrobial activity against pathogenic bacteria, and thus pBD2 could be a good candidate as a bactericidal agent for pigs. This study reported the successful recombinant expression of pBD2 in Escherichia coli and analysis of its(More)
Calcium influx triggers and accelerates endocytosis in nerve terminals and nonneuronal secretory cells. Whether calcium/calmodulin-activated calcineurin, which dephosphorylates endocytic proteins, mediates this process is highly controversial for different cell types, developmental stages, and endocytic forms. Using three preparations that previously(More)
Various fusion techniques have been used to treat lumbar spine isthmic spondylolisthesis (IS) in adults, including anterior lumbar interbody fusion (ALIF), posterior lumbar interbody fusion (PLIF), transforaminal lumbar interbody fusion (TLIF), posterolateral fusion (PLF), and circumferential fusion. The objective of this study was to evaluate which fusion(More)
OBJECTIVE The purpose of this study was to determine influences of cervical spine positions, turning times, and cervical segments on cadaver intradiscal pressure (IDP) during cervical spinal manipulative therapy (SMT). METHODS We simulated cervical SMT with stretching and rotation on 7 fresh adult cadaver specimens in the material test system machine. The(More)
As a powerful and promising analytical technique, laser-induced breakdown spectroscopy (LIBS) has been undergoing a rapid development in the past years. LIBS technique allows for rapid, multi-elemental, in situ measurements, and has been proposed for real time soil analysis. Currently, a global effort is focused on how to further improve the analytical(More)
The aim of the study was to evaluate the biomechanical performance and clinical results of TSRH’s Hook plus screw fixation after direct repair of spondylolysis defects in the pars interarticularis. Biomechanical testing of eight calf cadaver lumbar spines were used to provide comparative biomechanical data on TSRH’s Hook plus screw fixation, and for the(More)
Vesicle fusion with the plasma membrane generates an Ω-shaped membrane profile. Its pore is thought to dilate until flattening (full-collapse), followed by classical endocytosis to retrieve vesicles. Alternatively, the pore may close (kiss-and-run), but the triggering mechanisms and its endocytic roles remain poorly understood. Here, using confocal and(More)
OBJECTIVE To evaluate the biomechanical effect and clinical results of hook screw fixation after direct repair of lumbar spondylous defects in the pars interarticularis. METHODS L(2)-L(6) spines of 8 fresh-frozen and thawed calf cadavers were used for mechanical testing. Bilateral spondylous defects were created in the L(4)vertebra. The intervertebral(More)
OBJECTIVE To compare the immediate stability of different fixation techniques for the direct repair of spondylolytic defects in the pars interarticularis. METHODS Eight fresh-frozen and thawed calf cadaveric lumbar spines were used for mechanical testing. A modified Scott, screw-rod-hook, screw-rod construct fixation and Buck techniques were applied to(More)