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be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders. This document is the postprint version of the journal article. Some differences between the(More)
Porcine circovirus type 2 (PCV2) is considered to be associated with post-weaning multisystemic wasting syndrome (PMWS), which is a newly emerged economically important swine disease. The entire coding region of open reading frame 2 (ORF2), encoding the viral capsid protein (Cap), of PCV2 was cloned and sequenced from the clinical specimen obtained from(More)
Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated diseases in pigs. The sole structural capsid protein of PCV2, Cap, consists of major antigenic domains, but little is known about the assembly of capsid particles. The purpose of this study is to produce a large amount of Cap protein using Escherichia coli(More)
Porcine circovirus type 2 possesses a circular, single-stranded DNA genome that requires the replication protein (Rep) for virus replication. To characterize the DNA binding potential and the significant region that confers the nuclear localization of the Rep protein, the defined coding regions of rep gene were cloned and expressed. All of the recombinant(More)
Ensuring consensus on QoS attributes is imperative for the discovery of Web services. Inconsistent views of service providers and consumers on QoS attributes could be problematic, even though they have consensus on characteristics. For instance, they may have distinct views of the QoS attribute of service reliability, wherein a consumer considers that a(More)
Appropriate use of group consensus on service consumers' QoS opinions can improve web service discovery. For web service participants with different backgrounds or preferences it may not be easy to reach a consensus on the Web service QoS opinions, so they should be treated as multi-groups. Also, to prevent possibly useful opinions from being omitted(More)
a r t i c l e i n f o a b s t r a c t QoS has been considered as a significant factor for web service marketing and selection. The interpretation of QoS value from web service consumers and providers would be very different. However, a large group of web service participants with different backgrounds may have difficulties in reaching consensus on the(More)
Little is known of how the genetic background effects the phenomenon of genomic imprinting. The H19 gene belongs to a cluster of imprinted genes on human chromosome 11. Here we show that the alternative splicing of a human H19 transcript is genotype-specific. Moreover, this variant transcript, which lacks exon 4, is either not found at all, is widely(More)
Glycine N-methyltransferase (GNMT) is a folate binding protein commonly diminished in human hepatoma yet its role in tumor development remains to be established. GNMT binds to methylfolate but is also inhibited by it; how such interactions affect human carcinogenesis is unclear. We postulated that GNMT plays a role in folate-dependent methyl group(More)
Mu transposition recombination technique was firstly used as a mutagenesis tool to explore a cluster of genes involved in biosynthesis and regulation of pigments in P. aeruginosa. Eight pigment mutants were screened and identified. Gene cloning and sequencing of the region flanking the insertion revealed that the genes hmgA, ptsP, sucC, phzS, phzF1 were(More)