Wayne R Springer

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All three forms of discoidin I, an endogenous N-acetylgalactosamine-binding lectin from D. discoideum, contain the amino acid sequence gly-arg-gly-asp also found in fibronectin and implicated in its attachment to cells. Synthetic peptides containing these and adjacent amino acids of discoidin I block organized streaming during aggregation of D. discoideum(More)
Partially differentiated cells of two species of cellular slime molds, Dictyostelium discoideum and Dictyostelium purpureum, were labeled with isothiocyanate derivatives of fluorescent dyes. The labeled cells of each species segregated promptly when mixed and placed on moist filters. We determined whether cells studied at a time when they demonstrated this(More)
Identification of cell surface molecules that play a role in cell-cell adhesion (here called cell adhesion molecules) has been achieved by demonstrating the inhibitory effect of univalent antibodies that bind these molecules in an in vitro assay of cell-cell adhesion. A more convenient reagent, intact (divalent) antibody, has been avoided because it might(More)
This dissertation has been read by each member of the following supervisory committee and by majority vote has been found to be satisfactory. in its final form and have found that (1) its format, citations, and bibliographic style are consistent and acceptable; (2) its illustrative materials including figures, tables, and charts are in place; and (3) the(More)
The amount of total endogenous cellular and cell surface lectin in aggregating Dictyostelium purpureum was determined by a number of immunochemical techniques. The results show that of the 5 x 106 molecules of the lectin (called purpurin) per aggregating cell only about 2% (1 x 105 molecules) is present on the cell surface. Cell surface purpurin can be(More)
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