Wayne Patterson

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Monoclonal antibodies were raised to a DNA.RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase. A monoclonal antibody with the highest affinity and specificity was selected. This antibody bound the DNA.RNA heteropolymer and poly(I).poly(dC) equally but 100-fold higher levels of(More)
A series of dipeptide derivatives of Rhodamine, each containing an arginine residue in the P1 position and one of ten representative benzyloxycarbonyl (Cbz)-blocked amino acids in the P2 position, has been synthesized, purified and characterized as substrates for serine proteinases. These substrates are easily prepared with high yields. Cleavage of a single(More)
  • Program Solicitation, Maria Burka, +12 authors Eva Zanzerkia
  • 2007
Preliminary Proposal Due Date(s) (required) (due by 5:00 p.m. proposer's local time): Each preliminary proposal must uniquely correspond to a Letter of Intent-see instructions. Full Proposal Submission Window Date(s) (due by 5:00 p.m. proposer's local time): By invitation only, based on review of preliminary proposal-see instructions. In furtherance of the(More)
Rapid, convenient and non-isotopic nucleic-acid hybridization methods are needed for this technology to have practical use in clinical diagnostic tests. A method for hybridization of RNA with a DNA probe in solution followed by capture and measurement of the hybrid is described. DNA probes complementary to 23S rRNAs from Escherichia coli and Bacillus(More)