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Action spectra were determined for lightinduced phase shifts of the circadian rhythm of adult emergence in Drosophila pseudoobscura. The action spectra for advance and delay phase shifts are similar; the most effective wavelengths are in the blue region, with a sharp cutoff above 500 nanometers. This similarity suggests that the same photoreceptive pigment(More)
Drosophila melanogaster was raised on aseptic diets, with and without beta-carotene. The sensitivity of visual receptors in the carotenoid-depleted flies was lowered 3 log units, but the photosensitivity of the circadian rhythm was not affected. This result suggests that the chromophore of the photopigment which mediates light effects on the circadian(More)
The antioxidant activities of compounds endogenous to mammalian rod outer segments (ROS) were investigated in vitro by measuring the oxidative loss of polyunsaturated fatty acids (PUFA's) from the membranes of intact ROS and from liposomes made from ROS phospholipids (PL) to which lipid soluble compounds had been added. The membranes were exposed to the(More)
The lysosome fractions from bovine retina, liver and retinal pigment epithelium were isolated by subcellular fractionation and compared with regard to the relative proportions of several hydrolytic enzyme activities. It was found that the lysosome fraction of the retinal pigment epithelium is more than three times as active as the lysosome fractions from(More)
Soluble proteins can be extracted by osmotic shock of purified rod (photoreceptor cell) outer segments that have intact plasma membranes. The soluble proteins include a component that contains tightly bound GDP-Exchange of this GDP with exogenous nucleotide is catalyzed by (and requires) the membranes from the outer segments. ATP does not participate in(More)