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Cytosolic sulfotransferases, which mediate activation and detoxification of both endogenous and exogenous compounds, consist of at least five different gene families (ST1 to ST5) in mammals. Several cDNAs corresponding to ST1A forms have been reported, but their functional properties are not well characterized. In addition, only a single form of ST1A(More)
Supplement of 1% lithocholic acid (LCA) in the diet for 5-9 days resulted in elevated levels of the marker for liver damage aspartate aminotransferase and alkaline phosphatase activities in both farnesoid X receptor (FXR)-null and wild-type female mice. The levels were clearly higher in wild-type mice than in FXR-null mice, despite the diminished expression(More)
Comparison of several recently determined X-ray crystal structures of mammalian cytochrome P450 family 2 enzymes suggests considerable movement of helix B' when ligands bind. To investigate the functional role of helix B' in P450 2B1, residues 100-109 were substituted with alanine and phenylalanine. Kinetic properties were examined with the typical 2B(More)
Since sulfation is the main metabolic pathway of troglitazone, accounting for about 70% of the metabolites detected in human plasma, we have aimed to identify human cytosolic sulfotransferases catalyzing the sulfation of troglitazone and to examine a possible role of the sulfation in the cytotoxicity observed in cell lines of human origin (HepG2 and Hep3B).(More)
The mRNA of a sulfotransferase (St2b2) mediating cholesterol sulfation was detected in mouse skin. Recombinant St2b2 also mediated the sulfation of pregnenolone, 3beta-hydroxy-5-cholen-24-oic acid, and dehydroepiandrosterone. St2b2 protein was detected in skin cytosols on Western blotting. The addition of 10 nM TPA to skin epidermal cells from newborn mice(More)
Although catecholamine sulfation is higher in the kidney than in the liver of mice, no detectable amounts of previously reported sulfotransferases (STs) such as St1a, St1b, St1c, and St1e were expressed in mouse kidney cytosols. A new sulfotransferase (St1d1) cDNA was isolated from kidney cDNA library of BALB/c strain by reverse transcription-polymerase(More)
PURPOSE The degree of crushing and intraoral food flow was evaluated using peanuts by the occlusal forms of a removable bridge as indices to clarify the occlusal formative effect of maxillary molars on masticatory function. METHODS The subject was a 29-year-old male without any gnathostomatic disorders and his removable bridge ([7] 6 [5]) was so made in(More)
PURPOSE Unilateral chewing requires a greater number of chewing strokes up to the point of the first swallowing. The rate of storage of ground food particles in the oral vestibule is said to be high during unilateral chewing. However, influence of the masticatory method, that is free-sided or unilateral chewing, involved in masticatory efficiency has not(More)
A three-part license expansion for omalizumab (Xolair(®)), humanized anti-IgE antibody, was recently made in Japan for paediatric use, additional higher doses and revised dosing frequency in allergic asthma. The dosing level and frequency of omalizumab are guided by a dosing table based on the total serum IgE and bodyweight. Nonlinear mixed-effect(More)
PURPOSE Various methods of evaluating the ability of mastication have been reported, most of which involve evaluating of the ability to comminute foods. In this study, a new method of evaluating the ability of mastication focusing on bolus formation was applied to denture wearers. METHODS One hundred and twenty-five subjects (71 males and 54 females)(More)
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