Wasiulla Rafi

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Drug-resistant tuberculosis is an increasing problem worldwide. There are few reports of drug susceptibility patterns of Mycobacterium tuberculosis isolated from cases of tuberculous meningitis. A 5-year retrospective study aimed at analysing the drug susceptibility profile of M. tuberculosis isolated from tuberculous meningitis cases was conducted. A total(More)
SETTING Tuberculous meningitis (TBM) is the commonest form of neurotuberculosis in the Indian subcontinent. Rapid laboratory confirmation of TBM is important for the institution of early treatment and to avoid associated morbidity and mortality. The polymerase chain reaction (PCR) is the most widely applied alternative rapid diagnostic technique for TBM. (More)
A PCR was standardized for amplifying three different mycobacterial--IS6110, MPB-64, 65 kDa DNA sequences. A comparative evaluation of the three PCR assays was carried out for the rapid diagnosis of tuberculous meningitis (TBM) using cerebrospinal fluid (CSF) specimens. While the IS6110 PCR was a single-step amplification reaction, the MPB-64 and 65 kDa(More)
PURPOSE To evaluate the role of the radiometric BACTEC 460TB system and the conventional Lowenstein-Jensen (LJ) medium for isolation of M. tuberculosis from cerebrospinal fluid (CSF) samples of tuberculous meningitis (TBM) patients. METHODS CSF specimens (n=2325) from suspected TBM patients were processed for isolation of mycobacteria by inoculating(More)
Sickle cell disease is an inherited disorder that presents with a diverse group of clinical syndromes. Dr James Herrick first reported the disease in the year 1910. Ingram identified the substitution of valine for glutamic acid as the sixth amino acid of the beta globin chain. Since then amultitude of amino acid substitutions occurring at different regions(More)
BACKGROUND Acute bacterial meningitis is a significant cause of morbidity and mortality throughout the world. It can be difficult to diagnose, as the symptoms and signs are often non-specific. STUDY OBJECTIVE To evaluate the performance of an in-house semi-nested polymerase chain reaction (PCR) assay targeting the 16S rRNA gene of Eubacteria for the rapid(More)
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