- Full text PDF available (12)
1. A method is given for the concentration and purification of the soluble specific substance of the pneumococcus. 2. The material obtained by this method is shown to consist mainly of a carbohydrate which appears to be a polysaccharide built up of glucose molecules. 3. Whether the soluble specific substance is actually the polysaccharide, or occurs merely… (More)
The fundamental studies of Landsteiner and his coworkers (1) on complex antigens have established the important principle that the introduction into the protein molecule of a simple non-protein radical confers a new immunological specificity on the antigenic compound. Furthermore, this newly acquired specificity has been shown to depend upon the nature of… (More)
The soluble specific substance of Pneumococcus Type I has been chemically isolated from the bacterial cells and from autolyzed cultures as an acetyl polysaccharide. So far as could be determined by the methods employed, the acetyl polysaccharide in highly purified form absorbs from Type I antipneumococcus serum all demonstrable type-specific precipitins,… (More)
Pneumococci contain a non-protein constituent which, on the basis of its chemical and immunological properties, appears to be a carbohydrate distinct from the type-specific carbohydrate and common to the species.
The chemical and immunological properties of the acetylated and unacetylated forms of the p-aminophenol beta-glucoside of glucose have been described. The serological specificity of these beta-glucosides in combination with protein has been correlated with known changes in chemical structure and has been compared with the immunological properties of the… (More)
1. By partial acid hydrolysis a specific carbohydrate may be isolated from gum arabic (gum acacia). This carbohydrate is comparable in its precipitating activity for Type II (and Type III) antipneumococcus serum with the bacterial soluble specific substances themselves. 2. On hydrolysis this fraction yields galactose and two or more complex sugar acids, one… (More)
There can be but little doubt that the bactericidal activity of the colieine K which is elaborated by F~cherichia coli K235 resides in the protein portion of the bacterial somatic antigen (1). In its native state this component is firmly bound to a lipopolysaccharide and the two cannot be separated by the usual chemical or physical techniques. The question… (More)
1. A method is given for the isolation of a specifically reacting nitrogen-free polysaccharide from the so called E strain of Friedländer's bacillus. 2. The properties of this polysaccharide are described.
1. Autolysis of Pneumococcus is accompanied by proteolysis, which results in an increase in amino and non-coagulable nitrogen. 2. Autolysis of Pneumococcus is accompanied by lipolysis during which there is a liberation of ether-soluble fatty acids. 3. When extracts containing the active intracellular enzymes are added to heat-killed pneumococci, lysis of… (More)