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PURPOSE To develop and validate a rapid method for determining the dissociation constants with which pharmaceutical candidates and drugs bind to serum albumin and to alpha1-acid glycoprotein with the goal of deducing the extent of binding. METHODS The quenching of the intrinsic tryptophan fluorescence of serum albumin and alpha1-acid glycoprotein was(More)
PURPOSE This study investigated the intra-observer and inter-observer variation of Hertel exophthalmometry. METHODS Seventy normal Chinese adults had bilateral exophthalmometry performed by two examiners. The two examiners were masked to the measurement results. One examiner did the measurement twice and the other examiner measured it once; the order was(More)
A thin-layer chromatographic method for the simultaneous screening and confirmation of methadone and its primary metabolite (2-ethylidene-1,5-diemthyl-3,3-diphenylpyrrolidine) in urine specimens is presented. Urine is made basic and extracted with organic solvent. After separation and concentration, the residue is spotted, half on each of two thin-layer(More)
Twenty-six different opiates were analyzed by radioimmunoassay (RIA) at five different concentrations. At attempt is made to relate structural differences to the affinity of the compounds for the Roche RIA morphine antibody. The effects of substituent placement on the morphine molecule are studied. As expected, the basic 5-ring opiate structure is essential(More)
A method suitable for large scale screening and confirmation of urine speciments for amphetamine, methamphetamine, methadone, and its primary metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenlypyrrolidine) is described. The drugs are extracted from alkaline urine into an organic solvent. The amphetamine drugs are then back-extracted into a small volume of(More)
A rapid, sensitive, and specific procedure is described for the mass screening and confirmation of codeine and morphine in urine specimens. The method is sensitive to 0.5-mug/ml levels of both opiates in free and/or conjugate forms. The raw urine is screened directly by radiommunoassay, which is reactive to both free and glucuronide forms of codeine and(More)
This procedure positively identifies codeine and morphine in urine. Urine samples are hydrolyzed and extracted with organic solvent, and the extracts are evaporated and acetylated. The presence of codeine and morphine is ascertained by gas chromatography (3% OV-25 and 3% Poly-A 103 columns) and confirmed by thin-layer chromatography (system: ethyl(More)
Three solvent systems for thin-layer chromatography have been developed for methadone and its primary metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine). These solvent systems separate methadone and its primary metabolite, and are not interfered by other drugs of abuse in urine specimens.
Thin-layer chromatographic procedures are presented for the positive identification of methodone, primary metabolite of methodone (2-ethylidine-1,5-dimethyl-3,3-diphenylpyrrolidine), propoxyphene, norpropoxyphene, cocaine, benzoylecgonine, methoqualone, and phencyclidine from urine specimens. Initial screening of specimens is done by developing plates in(More)