Wade P Cavender

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This study describes the development of a TaqMan real-time quantitative polymerase chain reaction (QPCR) technique using the heat-shock protein 70 (Hsp 70) and 18S ribosomal DNA (18S rDNA) sequences to identify Myxobolus cerebralis and attempt to quantify infection severity within rainbow trout fry Oncorhynchus mykiss. Rainbow trout for this study were(More)
—Two experiments were conducted in vivo with eggs of rainbow trout Oncorhynchus mykiss to compare the bactericidal ability of four common disinfectants. A third test compared bacterial abundance estimation methods for fish eggs (use of a vortex mixer for agitating an egg versus rolling the egg across a petri dish). In the first test, the number of colony(More)
Bacterial coldwater disease, caused by Flavobacterium psychrophilum, has lead to the loss of significant numbers of hatchery-reared salmonids. The bacteria can be spread from parent to progeny within contaminated sperm and ovarian fluid and can enter the egg during fertilization. The addition of antibiotics to diluents and water-hardening solutions could(More)
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