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A culture of the bacterivorous rotifer H. rosa was separated from other Eucaryota in activated sludge and then purified by passages from most representatives of the bacterial microflora. A fully axenic culture was finally obtained by use of a lysing buffer and certain antibiotics.
DNA synthesis was inhibited during embryogenesis of Habrotrocha rosa with mitomycin C and hydroxyurea. Inhibition of DNA replication in early stages of embryogenesis, at the beginning of organogenesis, just after cavitation of the stomodeum, resulted in a complete inhibition of further development. After this stage of embryogenesis development was… (More)