Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase
- X. Ping, Bao-Fa Sun, Yun‐Gui Yang
- 10 January 2014
The methyltransferase like 3 (METTL3)-containing methyltransferase complex catalyzes the N6-methyladenosine (m6A) formation, a novel epitranscriptomic marker; however, the nature of this complex…
Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage
- Jianping Zhang, Xiao-Lan Li, Xiao-Bing Zhang
- 20 February 2017
It is shown that a double cut HDR donor, which is flanked by single guide RNA (sgRNA)-PAM sequences and is released after CRISPR/Cas9 cleavage, increases HDR efficiency by twofold to fivefold relative to circular plasmid donors.
Tracing haematopoietic stem cell formation at single-cell resolution
- Fan Zhou, Xianlong Li, Bing Liu
- 18 May 2016
Haematopoietic stem cells (HSCs) are derived early from embryonic precursors, such as haemogenic endothelial cells and pre-haematopoietic stem cells (pre-HSCs), the molecular identity of which still…
Identification of functional cooperative mutations of SETD2 in human acute leukemia
- Xiaofan Zhu, F. He, Qianfei Wang
- 9 February 2014
This study provides compelling evidence for SETD2 as a new tumor suppressor by identifying a transforming MLL-NRIP3 fusion gene and biallelic mutations inSETD2 (encoding a histone H3K36 methyltransferase), which is a distinct epigenetic mechanism for leukemia development.
Mouse embryonic head as a site for hematopoietic stem cell development.
Leukemic marrow infiltration reveals a novel role for Egr3 as a potent inhibitor of normal hematopoietic stem cell proliferation.
A robust nonirradiated mouse model of human MLL-AF9 leukemia is used andGene expression profiling and further functional validation revealed that Egr3 was a strong limiting factor for the proliferative potential of HSCs, providing a molecular basis for the more tightened quiescence of H SCs in leukemia.
An abnormal bone marrow microenvironment contributes to hematopoietic dysfunction in Fanconi anemia
- Yuan Zhou, Yongzheng He, Feng-Chun Yang
- 1 June 2017
It is demonstrated that mice with double knockout of both Fancc and Fancg genes had decreased bone formation at least partially due to impaired osteoblast differentiation from mesenchymal stem/progenitor cells.
Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation
- Yingchi Zhang, Jingliao Zhang, Xiaofan Zhu
- 7 October 2016
Nucleic Acids Research
The first intronic mutations in the intron 1 GATA site (int-1-GATA) of 5-aminolevulinate synthase 2 (ALAS2) have been identified in X-linked sideroblastic anemia pedigrees and it is found that hemizygous deletion led to an embryonic lethal phenotype due to severe anemia resulting from a lack of ALAS2 expression.
Stem cell science on the rise in China.
Loss of ASXL1 in the bone marrow niche dysregulates hematopoietic stem and progenitor cell fates
- Peng Zhang, Zizhen Chen, Feng-Chun Yang
- 23 January 2018
This study shows that BMSCs derived from chronic myelomonocytic leukemia patients had reduced expression of ASXL1, which impaired the maintaining cord blood CD34+ cell colony-forming capacity with a myeloid differentiation bias, and provides mechanistic insight into the function of Asxl1 in the niche to maintain normal hematopoiesis.