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Food storage material silver nanoparticles interfere with DNA replication fidelity and bind with DNA.
TLDR
It is found that nanosilver materials bind with genomic DNA under atomic force microscopy, and this might be an explanation for the compromised DNA replication fidelity. Expand
Selection of Reference Genes for Quantitative Gene Expression Studies in Platycladus orientalis (Cupressaceae) Using Real-Time PCR
TLDR
This study selected 10 reference gene candidates from transcriptome data of P. orientalis and examined their expression profiles by qRT-PCR, and showed that ubiquitin-conjugating enzyme E2 (UBC) and alpha-tubulin (aTUB) were the optimum pair of reference genes at all developmental stages and under all stress conditions. Expand
Enhanced amplification of GC-rich DNA with two organic reagents.
TLDR
Two frequently seen and inexpensive reagents-ethylene glycol and 1,2-propanediol-were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp. Expand
Transdermal protein delivery by a coadministered peptide identified via phage display
TLDR
The short synthetic peptide, ACSSSPSKHCG, identified by in vivo phage display, facilitated efficient transdermal protein drug delivery through intact skin and enabled penetration of insulin into hair follicles beyond a depth of 600 μm. Expand
Polysaccharides from Ganoderma lucidum Promote Cognitive Function and Neural Progenitor Proliferation in Mouse Model of Alzheimer's Disease
TLDR
Oral administration of the polysaccharides and water extract from G. lucidum promoted neural progenitor cell proliferation to enhance neurogenesis and alleviated cognitive deficits in transgenic AD mice, suggesting that GLP could serve as a regenerative therapeutic agent for the treatment of cognitive decline associated with neurodegenerative diseases. Expand
Atomic force microscopy investigation of the characteristic effects of silver ions on Escherichia coli and Staphylococcus epidermidis.
TLDR
It is shown that AFM can provide new insight into the antimicrobial mechanism of silver ions, and show a new methodological approach to understand the relationship between structure and function of microbial cell when it was exposed to external stimulation. Expand
Genetic Diversity of the Critically Endangered Thuja sutchuenensis Revealed by ISSR Markers and the Implications for Conservation
TLDR
The low genetic differentiation among populations implies that the conservation efforts should aim to preserve all the extant populations of this endangered species. Expand
Interactions between μ-Opioid Receptor Agonists and Cannabinoid Receptor Agonists in Rhesus Monkeys: Antinociception, Drug Discrimination, and Drug Self-Administration
TLDR
Cannabinoid receptor agonist CP 55,940 and WIN 55,212 enhanced the antinociceptive effects but not the discriminative stimulus or positive reinforcing effects of μ-opioid receptor agonists in rhesus monkeys, supporting the view that combining cannabinoid and opioid receptor agonism might result in enhanced treatment effectiveness for pain without similarly enhancing abuse and dependence liability. Expand
Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles.
TLDR
It is demonstrated that the immobilized β-glucosidase has a good prospect in industrial applications and retained more than 90% of its initial activity after being operated for 15 successive batches. Expand
Quantitative Evaluation and Selection of Reference Genes for Quantitative RT-PCR in Mouse Acute Pancreatitis
TLDR
The expression levels of ACTB, TUBB, and B2M were found to be significantly upregulated during acute pancreatitis, whereas the expression level of 18sRNA was downregulated, and the combination of RPL-13A and YWHAZ was the most stable group of reference genes in the authors' experiments. Expand
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