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DNA vaccination with a gene encoding Toxoplasma gondii GRA6 induces partial protection against toxoplasmosis in BALB/c mice
BackgroundInfection with the protozoan Toxoplasma gondii causes serious public health problems and is of great economic importance worldwide. Protection from acute toxoplasmosis is known to beExpand
Seroprevalence and first multilocus microsatellite genotyping of Neospora caninum in dairy cattle in Henan, central China.
Neospora caninum is one of the important causes of abortion in cattle worldwide, and losses due to neosporosis to the cattle industry are considerable. However, the knowledge of geneticExpand
Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China
The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to JuneExpand
Pseudanoplocephala crawfordi is a member of genus Hymenolepis based on phylogenetic analysis using ribosomal and mitochondrial DNA sequences
Abstract Pseudanoplocephala crawfordi is one of the important zoonotic cestodes causing economic significance and public health concern. In the present study, the phylogenetic position of P.Expand
Simultaneous identification of three highly pathogenic Eimeria species in rabbits using a multiplex PCR diagnostic assay based on ITS1-5.8S rRNA-ITS2 fragments.
Eimeria stiedai, E. intestinalis, and E. flavescens are highly pathogenic in rabbits, especially rabbits younger than 3 months. In this study, the complete ITS1-5.8S rRNA-ITS2 sequences of six rabbitExpand
First molecular identification of Buxtonella ciliates from captive-bred mangabeys (Cercocebus torquatus) from China
Buxtonella species are large cyst-forming ciliates that infect ruminants and monkeys, and are morphologically similar to Balantidium coli ciliates that infect pigs, humans, monkeys, and otherExpand
Molecular Detection and Genotyping of Toxoplasma gondii and Neospora caninum in Slaughtered Goats in Central China.
Toxoplasma gondii and Neospora caninum infections can cause reproductive failure in animals, including goats, and toxoplasmosis is one of the most important foodborne diseases. However, informationExpand
Prevalence and molecular characterization of Sarcocystis infections of retail beef products from central China.
Cattle are the intermediate hosts for five Sarcocystis species including S. hominis and S. heydorni, which also infect humans. To investigate the prevalence of Sarcocystis infections in beef productsExpand
Effects of different inoculation routes on the parasitic sites of Cryptosporidium baileyi infection in chickens.
Cryptosporidiosis is prevalent in domesticated, caged, and wild birds. Cryptosporidium baileyi, an ascendant species of avian Cryptosporidium, is an important pathogen. It causes respiratory diseaseExpand
Multiplex PCR primer design for simultaneous detection of multiple pathogens.
  • W. Yan
  • Biology, Medicine
  • Methods in molecular biology
  • 2015
Multiplex PCR provides a powerful tool for simultaneous detection and discrimination of multiple pathogens or different subtypes of a causative agent from humans, animals, and plants in a singleExpand